Efficient reaction mechanism to detect lesion sites in DNA by repair enzymes
| Project/Area Number |
26810084
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Yazawa Kenjiro 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (70726596)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 全反射蛍光顕微鏡 / DNAハイブリダイゼーション / 一分子観察 / 水晶発振子マイクロバランス / アンサンブル / DNA修復酵素 / 蛍光色素 / ハイブリダイゼーション / 酸化ストレス / 電子伝達 |
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| Outline of Final Research Achievements |
DNA hybridization is a fundamental reaction for our lives. To date, ensemble measurement is the major stream to detect a hybridization reaction and little is known about the hybridiation at a single-molecule level. In this study, we monitored the hybridization of DNAs, including 8 mer, 12 mer, and PolyA-PolyT, at a single-molecule level by using total internal reflection microscopy. As a result, we found that the hybridization of 8 mer proceeded through a single step, whereas the hybridization of 12 mer and PolyA-PolyT proceeded through three multiple steps. In the case of 12 mer, the hybridization initialized with its end suspended. On the other hand, the hybridization of Poly-PolyT showed the dangling behavior and continued to attach or detach to each other. Therefore, single-molecule measurement in this study contributed to understand the details of the DNA hybridization, which will be of great use for the DNA-related reactions such as DNA repairing.
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Report
(3 results)
Research Products
(1 results)
