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Efficient reaction mechanism to detect lesion sites in DNA by repair enzymes

Research Project

Project/Area Number 26810084
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Yazawa Kenjiro  国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (70726596)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords全反射蛍光顕微鏡 / DNAハイブリダイゼーション / 一分子観察 / 水晶発振子マイクロバランス / アンサンブル / DNA修復酵素 / 蛍光色素 / ハイブリダイゼーション / 酸化ストレス / 電子伝達
Outline of Final Research Achievements

DNA hybridization is a fundamental reaction for our lives. To date, ensemble measurement is the major stream to detect a hybridization reaction and little is known about the hybridiation at a single-molecule level. In this study, we monitored the hybridization of DNAs, including 8 mer, 12 mer, and PolyA-PolyT, at a single-molecule level by using total internal reflection microscopy. As a result, we found that the hybridization of 8 mer proceeded through a single step, whereas the hybridization of 12 mer and PolyA-PolyT proceeded through three multiple steps. In the case of 12 mer, the hybridization initialized with its end suspended. On the other hand, the hybridization of Poly-PolyT showed the dangling behavior and continued to attach or detach to each other. Therefore, single-molecule measurement in this study contributed to understand the details of the DNA hybridization, which will be of great use for the DNA-related reactions such as DNA repairing.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2014

All Presentation (1 results)

  • [Presentation] 全反射蛍光顕微鏡を用いたDNA二重鎖形成の一分子動力学2014

    • Author(s)
      古澤 宏幸・矢澤 健二郎・岡畑 惠雄
    • Organizer
      第24回バイオ・高分子シンポジウム
    • Place of Presentation
      東京工業大学
    • Year and Date
      2014-07-25
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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