Periplasmic proton accumulation mechanism in alkaliphilic bacteria capable of extracellular electron transport

• Project/Area Number: 26810085
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: okamoto akihiro 東京大学, 工学(系)研究科(研究院), 助教 (70710325)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 好アルカリ細菌 / プロトン局在性 / 細胞外電子移動 / 微生物燃料電池 / 極限環境微生物 / 電気化学 / 細胞外シトクロム

Outline of Final Research Achievements

Alkaliphilic gram-positive microbe capable of extracellular electron transport (EET) was enriched and isolated from The Cedars spring at pH 12, using on-site microbial fuel cell system with anode connected with air cathode. Also, we developed a method to monitor the periplasmic proton concentration using the EET-capable microbe.

Research Products

• [Journal Article] Cation-limited kinetic model for microbial extracellular electron transport via an outer membrane cytochrome <i>C</i> complex (2016)
  • Author(s): Akihiro Okamoto, Yoshihide Tokunou, Junki Saito
  • Journal Title: Biophysics and Physicobiology Volume: 13 Issue: 0 Pages: 71-76
  • DOI: 10.2142/biophysico.13.0_71
  • NAID: 130005154008
  • ISSN: 2189-4779
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Extracellular Electron Transport Scarcely Accumulates Proton Motive Force in Shewanella oneidensis MR-1 (2015)
  • Author(s): Yoshihide Tokunou, Kazuhito Hashimoto and Akihiro Okamoto
  • Journal Title: BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN Volume: in press Issue: 5 Pages: 690-692
  • DOI: 10.1246/bcsj.20140407
  • NAID: 130005069513
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Electrical Interaction in Biofilms through Outer-Membrane Heme Proteins (2015)
  • Author(s): Akihiro Okamoto
  • Organizer: BIT's 6th World Gene Conference - 2015
  • Place of Presentation: Shangri-La Hotel (SLH), Qingdao, China
  • Year and Date: 2015-11-13
  • Int'l Joint Research / Invited
• [Presentation] Scarce Accumulation of Proton Motive Force in Shewanella oneidensis MR-1 During Extracellular Electron Transport (2015)
  • Author(s): Akihiro Okamoto, Yoshihide Tokunou, Kazuhito Hashimoto
  • Organizer: The 5th International Meeting on Microbial Electrochemistry and Technologies
  • Place of Presentation: Arizona State University, Tempe, AZ, USA
  • Year and Date: 2015-10-01
  • Int'l Joint Research