Synthetic study of cysteine rich proteins

Research Project

Project/Area Number	26810088
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Bio-related chemistry
Research Institution	Nagoya University
Principal Investigator	Oishi Shunsuke 名古屋大学, トランスフォーマティブ生命分子研究所, 助教 (80707795)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	有機合成化学 / タンパク質の化学合成 / ケミカルバイオロジー / 植物化学 / 環状ペプチド / 生理活性中分子 / タンパク質 / 有機合成 / ライゲーション / 生体関連物質
Outline of Final Research Achievements	We synthesized LURE pollen tube attracting proteins, which plays an important role in plant reproduction. The synthesis was accomplished by KAHA peptide ligation reaction of two peptide segments obtained by Fmoc solid phase peptide synthesis. We found that the ligation reaction proceed efficiently by addition of reducing agent (TCEP). We also synthesized cyclic peptides having partial sequence of LURE proteins and the cyclic peptides exhibited the same biological activity as the parent LURE proteins.