Functional molecular robot using RNA/RNP nanostructures

• Project/Area Number: 26810089
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Osaka University (2015-2016); Kyoto University (2014)
• Principal Investigator: Shibata Tomonori 大阪大学, 産業科学研究所, 特任助教(常勤) (80711960)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: RNA / タンパク質 / 核酸ナノテクノロジー / RNAナノテクノロジー / RNA-タンパク質複合体 / 核酸ナノ構造 / RNAナノ構造 / 分子ロボット / ナノマシン

Outline of Final Research Achievements

We designed and constructed RNA-protein complex-based functional molecular robots that exert desired funtions in response to proteins. The functional molecular robots consisting of RNA and protein can be constructed within live cells. We demonstrated that mammalian cell fate can be controlled using functional molecular robots. Construction of a molecular robot that functions in a particular cell will be useful for medical applications.

Research Products

• [Presentation] Synthetic RNA-protein nano machines that function in vitro and in live cell (2015)
  • Author(s): ○Shibata T, Suzuki Y, Sugiyama H, Endo M, Saito H
  • Organizer: ISNAC20
  • Place of Presentation: イーグレ姫路 (兵庫県 姫路市)
  • Year and Date: 2015-09-23
  • Int'l Joint Research
• [Presentation] タンパク質応答性RNAナノマシン (2015)
  • Author(s): ○柴田知範、鈴木勇輝、杉山弘、遠藤政幸、齊藤博英
  • Organizer: 日本RNA学会年会
  • Place of Presentation: ホテルライフォート札幌 (北海道 札幌市)
  • Year and Date: 2015-07-15
• [Presentation] A RNA-protein-based nanomachine for controlling functional RNAs (2014)
  • Author(s): Tomonori Shibata, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, Hirohide Saito
  • Organizer: The 41st International Symposium on Nucleic Acids Chemistry 2014
  • Place of Presentation: 北九州
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] Photo-regulation of RNA-protein-based nanostructures (2014)
  • Author(s): Tomonori Shibata, Kenzo Fujimoto, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, Masahiro Takinoue, Hirohide Saito
  • Organizer: 20th International Conference on DNA computing and Molecular Programming
  • Place of Presentation: 京都
  • Year and Date: 2014-09-22 – 2014-09-26
• [Book] Methods Mol Biol. (2015)
  • Author(s): Shibata T, Suzuki Y, Sugiyama H, Endo M, Saito H
  • Publisher: Humana Press
• [Book] Methods in Molecular Biology 1316 (Chapter 14) (2015)
  • Author(s): Tomonori Shibata, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, and Hirohide Saito
  • Publisher: Human press