| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The method for the construction of “covalently-linked RNA-peptide complex library” that has the randomized region both in RNA and peptide subunit was developed to perform the screening of the artificial RNP enzymes for the post-translational phosphorylation of targeted peptide and protein. We could establish the conditions to synthesize the RNP library by applying the mRNA display method. In addition, we could find the screening conditions for the construction of ATP-binding RNP receptors with high specificity for the pyrophosphate moiety of ATP through the verification of the effect of the conditions of the counter-SELEX to the specificity of receptors. Furthermore, we developed the post-modification method of the RNP receptors for the screening of the functional RNPs that are modified with fluorophores or catalytic groups. These results can be utilized for the rational screening of the artificial enzymes from the new RNP library.
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