| Project/Area Number |
26810094
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Konan University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 三重鎖 / フーグスティーン塩基対 / 分子クラウディング / イオン液体 / センサー / 四重鎖 / 修飾核酸 / 逆転写阻害 / DNAナノスイッチ / 一塩基変異 / DNAセンサー / 極限環境 / ワトソン・クリック塩基対 / 熱力学的解析 |
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| Outline of Final Research Achievements |
We investigated the stability for DNA duplexes, triplexes, and G-quadruplexes under ultimate environments such as high ionic conditions inducing hydrated ionic liquid (IL) of choline dihydrogen phosphate (choline dhp) and molecular crowding conditions inducing by polyethylene glycols. Our quantitative analysis demonstrated that DNA triplex formation is markedly stabilized in high ionic conditions and molecular crowding conditions compared with aqueous solution. Especially, in choline dhp, the stability of Hoogsteen base pairs in the triplexes is comparable to that of Watson-Crick base pairs. Moreover, a molecular beacon that forms a triplex with a conserved HIV-1 sequence was then designed and tested in choline dhp. The molecular beacon specifically detected the target duplex via triplex formation with 10,000-fold sequence selectivity. The unique behaviors of DNAs under ultimate environments will be useful in the design of oligonucleotides for use in nanomaterials.
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