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Development of dihydropyrimidine fluorescent probe to detect reactive oxygen species for analysis of in vivo oxidation

Research Project

Project/Area Number 26810095
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Research InstitutionYasuda Women's University

Principal Investigator

NISHIMURA Yoshio  安田女子大学, 薬学部, 講師 (60431516)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsジヒドロピリミジン / ピリミジン / 蛍光プローブ / 活性酸素 / 酸化 / ヘテロ環
Outline of Final Research Achievements

Development of a new method to detect reactive oxygen species (ROS) in high selectivity and sensitivity for analysis of in vivo oxidation state is a significant research theme. For this purpose, we set up our goal to establish a novel and practical dihydropyrimidine fluorescent probe that is oxidized by ROS to give strongly fluorescent pyrimidine.
In this project, we developed novel methods to synthesize hitherto unavailable 4,6-unsubstituted 2-aminodihydropyrimidines 5-carboxylic acid ethyl esters, 4,6-unsubstituted 5-acyl-2-phenyldihydropyrimidines and 4,6-unsubstituted 5-acyl-2-aminodihydropyrimidines, which expanded dihydropyrimidine-based chemistry.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Synthesis of 4,6-Unsubstituted 2-Aminodihydropyrimidine-5-Carboxylates via Sequential Staudinger/Aza-Wittig/Cyclization Reactions2015

    • Author(s)
      Y. Nishimura, H. Cho
    • Journal Title

      Synlett

      Volume: (in press) Issue: 02 Pages: 233-237

    • DOI

      10.1055/s-0034-1378932

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression and Substrate Range of <i>Streptomyces</i> Vanillate Demethylase2014

    • Author(s)
      Motohiro Nishimura, Yoshio Nishimura, Chinatsu Abe, and Mayuko Kohhata
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 37 Issue: 9 Pages: 1564-1568

    • DOI

      10.1248/bpb.b14-00337

    • NAID

      130004057438

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Weinrebアミド基を利用する 4,6-無置換 2-アリール-5-アシルジヒドロピリミジン誘導体の収束的合成2016

    • Author(s)
      西村良夫、長秀連
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Weinrebアミド基を利用する4,6-無置換 5-アシルジヒドロピリミジンの合成2015

    • Author(s)
      西村良夫、長秀連
    • Organizer
      第45回複素環化学討論会
    • Place of Presentation
      早稲田大学国際会議場
    • Year and Date
      2015-11-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 連続的 Staudinger/Aza-Wittig/環化反応による 4,6-無置換 2-アミノジヒドロピリミジン-5-エステルの合成2015

    • Author(s)
      西村良夫、長秀連
    • Organizer
      日本薬学会第 135 年会
    • Place of Presentation
      神戸学院大学(神戸)
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 新しい糖尿病合併症治療薬ラニレスタットの合成研究2015

    • Author(s)
      西村良夫
    • Organizer
      第 132 回日本薬学会中国四国支部例会
    • Place of Presentation
      安田女子大学(広島)
    • Year and Date
      2015-01-10
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 連続的Aza-Wittig/環化反応による4,6-無置換ジヒドロピリミジンの合成2014

    • Author(s)
      西村良夫、長秀連
    • Organizer
      第 44 回複素環化学討論会
    • Place of Presentation
      札幌市民ホール(札幌)
    • Year and Date
      2014-09-10 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] リパーゼによる desymmetrization を利用した N-Cbz (S)-α-メチルセリンの実用的合成プロセスの開発2014

    • Author(s)
      西村良夫、雲地早紀、久常由加里、岡水涼、森川瞳、西博行、生中雅也
    • Organizer
      日本プロセス化学会 2014 サマーシンポジウム
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2014-07-31 – 2014-08-01
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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