Enhanced Cell Evaluation by Phase Decomposition

• Project/Area Number: 26820086
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Osaka University
• Principal Investigator: TSAI CHIA-HUNG 大阪大学, 工学(系)研究科(研究院), 講師 (20647490)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: cell deformability / microfluidics / red blood cell / disease diagnosis / pressure sensor / lab on a chip

Outline of Final Research Achievements

A new microfluidic system to evaluate pure cell stiffness is developed using phase decomposition method. Investigations on the diseases of diabetes and multiple myoloma had been performed. Significant difference, such as deformability distribution, between patients and normal people are found.

Research Products

• [Journal Article] Cell Pinball: Phenomenon and Mechanism of Inertia-Like Cell Motion in a Microfluidic Channel (2016)
  • Author(s): C. Tsai and M. Kaneko
  • Journal Title: Biomicrofluidics Volume: 10 Issue: 2 Pages: 024116-024116
  • DOI: 10.1063/1.4945412
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cell Pinball: Phenomenon and mechanism of inertia-like cell motion in a microfluidic channel (2015)
  • Author(s): R. Murakami, C. Tsai, M. Kaneko, S. Sakuma, and F. Arai
  • Journal Title: Lab on a Chip Volume: 15 Issue: 16 Pages: 3307-3313
  • DOI: 10.1039/c5lc00535c
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] On-Chip Actuation Transmitter for Enhancing the Dynamic Response of Cell Manipulation using a Macro-scale Pump (2015)
  • Author(s): T. Monzawa, M. Kaneko, C. Tsai, S. Sakuma, and F. Arai
  • Journal Title: Biomicrofluidics Volume: 9 Issue: 1 Pages: 014114-014114
  • DOI: 10.1063/1.4907757
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Geometrical Alignment for Improving Cell Evaluation in a Microchannel with Application on Multiple Myeloma Red Blood Cells (2014)
  • Author(s): C. Tsai, S. Sakuma, F. Arai, T. Taniguchi, T. Ohtani, Y. Sakata and M. Kaneko
  • Journal Title: RSC Advances Volume: 4 Issue: 85 Pages: 45050-45058
  • DOI: 10.1039/c4ra08276a
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Frequency Responses of On-Chip Pressure Sensor with Different Deformation Chambers (2015)
  • Author(s): C. Tsai and M. Kaneko
  • Organizer: International Symposium on Micro-NanoMechatronics and Human Science (MHS2015)
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2015-11-23
  • Int'l Joint Research
• [Presentation] Pressure Transmitter for Local Pressure Sensing in a Microchannel (2015)
  • Author(s): C. Tsai and M. Kaneko
  • Organizer: IEEE International Conference on Nano/Molecular Medicine and Engineering (NANOMED2015)
  • Place of Presentation: Waikiki, USA
  • Year and Date: 2015-11-15
  • Int'l Joint Research
• [Presentation] On-Chip Micro Manometer (2015)
  • Author(s): C. Tsai and M. Kaneko
  • Organizer: International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS15)
  • Place of Presentation: Gyeongju, Korea
  • Year and Date: 2015-10-25
  • Int'l Joint Research
• [Presentation] An On-Chip, Electricity-Free and Single-Layer Pressure Sensor for Microfluidic Applications (2015)
  • Author(s): C. Tsai, T. Nakamura and M. Kaneko
  • Organizer: IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS2015)
  • Place of Presentation: Hamburg, Germany
  • Year and Date: 2015-09-28
  • Int'l Joint Research
• [Presentation] Novel Microfluidic Chip for Extracting Cell Deformability (2015)
  • Author(s): C. Tsai, K. Mizoue, M. Kaneko, S. Sakuma and F. Arai
  • Organizer: IEEE International Conference on Mechatronics and Automation (ICMA2015)
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-08-02
  • Int'l Joint Research
• [Presentation] Characteristics of Vision-Based On-Chip Pressure Sensor with Different Concentrations of Sensing Fluid (2015)
  • Author(s): Frederike Hesse, C. Tsai and M. Kaneko
  • Organizer: IEEE International Conference on Mechatronics and Automation (ICMA2015)
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-08-02
  • Int'l Joint Research
• [Presentation] On-Chip Pressure Sensing by Visualizing PDMS Deformation using Microbeads (2015)
  • Author(s): Chia-Hung Tsai
  • Organizer: The 28th IEEE Conference on Micro Electro Mechanical Systems (MEMS 2015)
  • Place of Presentation: Estoril Centro De Congressos, Estoril, Portugal
  • Year and Date: 2015-01-18 – 2015-01-22
• [Presentation] Improving the Evaluation of Cell Deformability by Different Channel Width in a Microfluidic Device (2014)
  • Author(s): Chia-Hung Tsai
  • Organizer: The 11th Int. Conf. on Ubiquitous Robots and Ambient Intelligence (URAI14)
  • Place of Presentation: Double Tree Hotel, Kuala Lumpur, Malaysia
  • Year and Date: 2014-11-12 – 2014-11-15
• [Presentation] What is the Difference of Cell Deformation between PUSH and PULL? (2014)
  • Author(s): Shinya Sakuma
  • Organizer: Proceeding of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS14)
  • Place of Presentation: Henry B. Gonzalez Convention Center, San Antonio, USA
  • Year and Date: 2014-10-26 – 2014-10-30