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Simultaneous high-frame-rate recognition of cells fast-flowing in microchannels toward ultra-fast cell sorting

Research Project

Project/Area Number 26820158
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Measurement engineering
Research InstitutionHiroshima University

Principal Investigator

Gu Qingyi  広島大学, 工学(系)研究科(研究院), 特任准教授 (30713979)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsHigh-speed vision / Cell sorting / Hardware implementation / frame-straddling camera / lab-on-a-chip / cell-based labeling / high speed vision
Outline of Final Research Achievements

In this research project, we have achieved high-frame-rate recognition of cells fast-flowing in microchannels toward ultra-fast cell sorting by implementing a parallel multi-object feature extraction algorithm on a frame-straddling high-speed vision platform. Image-based cell features can be extracted in real-time by using hardware circuits. Recognition and classification of cells can be performed in real-time. High resolution ROI regions of fast-flowing cells in microchannels could be stored and analyzed at 1000 fps or higher. And ultra-fast analysis, inspection, and cell sorting could be achieved to increase production efficiency in bio-assembling. Lab-on-a-chip practitioners can easily apply it to real-time cell sorting of cells fast-flowing in microchannels, such as red blood or iPS cells.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] LOC-Based high-throughput cell morphology analysis system2015

    • Author(s)
      Qingyi Gu, Tomohiro Kawahara, Tadayoshi Aoyama, Takeshi Takaki, Idaku Ishii, Ayumi Takemoto, Naoaki Sakamoto
    • Journal Title

      IEEE Transactions on Automation Science and Engineering

      Volume: 12 Issue: 4 Pages: 1346-1356

    • DOI

      10.1109/tase.2015.2462118

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous Vision-Based Shape and Motion Analysis of Cells Fast-Flowing in a Microchannel2013

    • Author(s)
      Qingy Gu, Tadayoshi Aoyama, Takeshi Takaki, Idaku Ishii
    • Journal Title

      IEEE Transactions on Automation Science and Engineering

      Volume: - Issue: 1 Pages: 204-215

    • DOI

      10.1109/tase.2013.2292583

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Real-Time LOC-based Morphological Cell Analysis System Using High-Speed Vision2014

    • Author(s)
      Qingyi Gu, Tadayoshi Aoyama, Takeshi Takaki, Idaku Ishii, Ayumi Takemoto, and Naoaki Sakamoto
    • Organizer
      Proc. Of the 2014 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS2014)
    • Place of Presentation
      Chicago, Illinois, USA
    • Year and Date
      2014-09-15
    • Related Report
      2014 Research-status Report
  • [Presentation] Rapid Vision-based Shape and Motion Analysis System for Fast-flowing Cells in a Microchannel2014

    • Author(s)
      Qingyi Gu, Tadayoshi Aoyama, Takeshi Takaki, and Idaku Ishii
    • Organizer
      Proc. of the 2014 IEEE International Conference on Robotics and Automation (ICRA2014)
    • Place of Presentation
      Hong Kong, China
    • Year and Date
      2014-06-04
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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