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Establishment of a digital PCR-based genotyping method for noroviruses in water

Research Project

Project/Area Number 26820222
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Civil and environmental engineering
Research InstitutionKyoto University

Principal Investigator

Hata Akihiko  京都大学, 工学研究科, 特別研究員(PD) (70726306)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords健康関連微生物 / ノロウイルス / Real-time PCR / デジタルPCR / 水系感染症 / 水環境 / リアルタイムPCR / 次世代シーケンシング / 腸管系ウイルス / 分子生物学
Outline of Final Research Achievements

It is known that genetically broad types of noroviruses are exist together in the water environment. Quantification of single genotype of noroviruses seems to be important to understand characteristics of noroviruses, in view of infection risk via water and epidemiology, more deeply. The aim of the study was to establish a method for simultaneous quantification of “whole norovirus” and “a genotype of norovirus” using the novel digital PCR platform.
Water samples in Japan were continuously collected and analyzed to determine epidemic norovirus genotypes. Based on nucleotide sequences of the epidemic types and those on the database, fluorescent probes, which react to each epidemic genotype specifically, were designed. In the conventional real-time PCR platform, the fluorescent probes worked ideally, while they resulted in non-specific signal in the digital PCR platform. The results imply that further improvement is necessary for establishing the assay.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2017 2016

All Presentation (2 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] Two-year monthly study on viruses in lake water polluted by wastewater effluents2017

    • Author(s)
      A. Hata, Y. SHirasaka, M. Ihara, N. Yamashita, H. Tanaka
    • Organizer
      19th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      アメリカ
    • Year and Date
      2017-05-15
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ILLUMINA-TING THE PREVALENCE OF GENETICALLY DIVERSE ASTROVIRUSES, INCLUDING NOVEL MLB- AND VA-CLADES, IN ENVIRONMENTAL WATER SAMPLES2016

    • Author(s)
      A. Hata, M. Kitajima, M. Ihara, C.P. Gerba, H. Tanaka
    • Organizer
      5th Food and Environmental Virology Conference
    • Place of Presentation
      日本
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2018-03-22  

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