Evaluation of tumor penetration ability of nanoparticles using 3D cell culture system toward particle design optimization
Project/Area Number |
26820356
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biofunction/Bioprocess
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Research Institution | The University of Tokyo |
Principal Investigator |
Ohta Seiichi 東京大学, 医学(系)研究科(研究院), 助教 (40723284)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | ナノ粒子 / 腫瘍浸透性 / スフェロイド / ヒアルロン酸 / ナノゲル / ドラッグデリバリー / 固形がん浸透性 / シリコン / 量子フドット / ドラックデリバリー |
Outline of Final Research Achievements |
Multicellular 3D cancer spheroid was fabricated as a tumor model using MKN74 human gastric cancer cells. Penetration behavior of model nanoparticles, hyaluronan nanogels incorporating anti-cancer drug cisplatin, into tumor spheroid was successfully visualized. It was found that the penetration ability of hyaluronan nanogels was significantly higher than that of their linear analog, suggesting the importance of the shape of drug nanocarriers. Furthermore, a mathematical model based on reaction-diffusion equation was proposed to describe the penetration behavior of nanoparticles. The model calculation fitted the experimental results well.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Production of Cisplatin-Incorporating Hyaluronan Nanogels via Chelating Ligand-Metal Coordination2016
Author(s)
S. Ohta, S. Hiramoto, Y. Amano, M. Sato, Y. Suzuki, M. Shinohara, S. Emoto, H. Yamaguchi, H. Ishigami, Y. Sakai, J. Kitayama, and T. Ito
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Journal Title
Bioconjugate Chemistry
Volume: 27
Issue: 3
Pages: 504-508
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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