| Project/Area Number |
26820358
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Oshiba Yuhei 東京工業大学, 資源化学研究所, 助教 (10708530)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | コンジュゲート / P450cam / P420 / 酵素活性 / 構造変化 |
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| Outline of Final Research Achievements |
In this study, the relationship between enzyme activity and structural changes of P450cam after its modification was evaluated with compounds of different properties. We revealed the existence of a new mode for P450 activity decrease after conjugation, which was distinct from a well-known inactivation mode for the conversion of P450 to the inactive P420 form. In this new mode, the activity decreased due to a reduction in binding or accessibility of a substrate to the heme pocket of P450cam. This obtained knowledge will help to guide designs for the allosteric regulation of P450cam activity for industrial and biotechnological applications.
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