Development of a nanostructured hybrid antibody molecule with DNA scaffold

Research Project

Project/Area Number	26820360
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Biofunction/Bioprocess
Research Institution	Nagoya University
Principal Investigator	Kato Teruyo 名古屋大学, 生命農学研究科, 研究員 (40727640)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	学会発表
Outline of Final Research Achievements	Antibodies have become essential tool in the life technology fields and their applications as a high-sensitivity sensor are increasing. In this research, we have developed a novel sensor molecule named ‘Hybribody’ which consists of DNA (PCR product or plasmid), DNA-binding protein, and antibody (scFv). First, we established an efficient production scheme for fusion proteins of DNA-binding protein and scFv using E. coli expression systems with an N-teminal tag and chaperon co-expression. Bivalent antibody-DNA hybrid molecule, Hybribody, was formed by mixing with the purified proteins and DNA fragments.