| Project/Area Number |
26820362
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kobe University |
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Principal Investigator |
Ishii Jun 神戸大学, 自然科学系先端融合研究環重点研究部, 准教授 (40512546)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 遺伝子スイッチ / ゲノム編集 / Gタンパク質シグナル / Gタンパク質共役型受容体 / 二量体形成 / 酵母 / セロトニン受容体 / アドレナリン受容体 / 遺伝子発現スイッチ / 発現スイッチ / レポーター遺伝子 |
|---|
| Outline of Final Research Achievements |
As the switching genome editing technology that can respond to G-protein signaling, we established the method permitting the pop-out of a gene and the alternative expression of another gene based on the Cre/loxP recombination technique. Herewith, it has enabled to induce concurrently “ON to OFF” and “OFF to ON” of two genes in concert with the detection of G-protein signaling. Using this technology, we developed the system for switching two gene expressions in response to the signal for dimer formation of G-protein coupled receptors (GPCRs); thereby, successfully detecting the homodimer formations of yeast endogenous Ste2 receptor and human serotonin receptor (HTR1A), and the heterodimer formation of human adrenergic receptor (ADRB2).
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