Analysis of mechanisms regulating memory consolidation and forgetting

• Project/Area Number: 26830003
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Chiba University
• Principal Investigator: Tonoki Ayako 千葉大学, 大学院薬学研究院, 講師 (90645425)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 老化 / 記憶 / 忘却 / ショウジョウバエ / ドーパミン / ニューロペプチド / 報酬記憶 / 罰記憶 / 味覚神経細胞

Outline of Final Research Achievements

The formation of memory declines with advancing age. However, susceptibility to memory impairments depends on the robustness of memory. In this study, we aimed to reveal how age-dependent changes in neural circuits affect memory formation by using Drosophila genetics and behavior analysis. We succeeded to show that memory declines are caused by age-dependent changes in neural circuits including a subset of dopaminergic neurons and neuropeptides expressing neurons.

Research Products

• [Journal Article] Successive and discrete spaced conditioning in active avoidance learning in young and aged zebrafish. (2017)
  • Author(s): Yang P, Kajiwara R, Tonoki A, Itoh M.
  • Journal Title: Neurosci Res. Volume: S0168-0102(17) Pages: 30502-3
  • DOI: 10.1016/j.neures.2017.10.005
  • Peer Reviewed / Open Access
• [Journal Article] ge-Related Changes in Insulin-like Signaling Lead to Intermediate-Term Memory Impairment in Drosophila. (2017)
  • Author(s): Tanabe K, Itoh M, Tonoki A.
  • Journal Title: Cell Reports Volume: 18(7) Issue: 7 Pages: 1598-1605
  • DOI: 10.1016/j.celrep.2017.01.053
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mib1 modulates dynamin 2 recruitment via Snx18 to promote Dll1 endocytosis for efficient Notch signaling. (2016)
  • Author(s): Okano M, Matsuo H, Nishimura Y, Hozumi K, Yoshioka S, Tonoki A, Itoh M
  • Journal Title: Genes Cells Volume: 28 FEB Issue: 5 Pages: 1-17
  • DOI: 10.1111/gtc.12350
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Aging impairs protein-synthesis-dependent long-term memory in Drosophila. (2015)
  • Author(s): Tonoki A, Davis RL.
  • Journal Title: J Neurosci. Volume: 35(3) Issue: 3 Pages: 1173-1180
  • DOI: 10.1523/jneurosci.0978-14.2015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Caspase inhibition in select olfactory neurons restores innate attraction behavior in aged Drosophila. (2014)
  • Author(s): Chihara, T., Kitabayashi, A., Morimoto, M., Takeuchi, K., Masuyama, K., Tonoki, A., Davis, R.L., Wang, J.W., and Miura, M.
  • Journal Title: PLOS Genetics Volume: 10 Issue: 6 Pages: e1004437-e1004437
  • DOI: 10.1371/journal.pgen.1004437
  • NAID: 120006799366
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 生存利益を伴う報酬記憶は加齢に対して頑強性を示す (2017)
  • Author(s): 殿城 亜矢子、小笠原 美奈、于 智華、伊藤 素行
  • Organizer: 第40回日本神経科学会大会
• [Presentation] NO-sGC pathway potentially contributes to age-related memory impairment in Drosophila (2017)
  • Author(s): Ayako Tonoki, Saki Nagai, Zhihua Yu, Hiroki Takahashi, Motoyuki Itoh
  • Organizer: 第10回分子高次機能研究会
• [Presentation] ショウジョウバエにおける記憶とNotchシグナルの関係の解明 (2017)
  • Author(s): 小林航、吉岡佐保、永井咲紀、粟崎健、伊藤素行、殿城亜矢子
  • Organizer: 第10回分子高次機能研究会
• [Presentation] 老化に伴う神経回路の長期的変化による味覚感受性の応答 (2017)
  • Author(s): 殿城亜矢子
  • Organizer: 次世代脳プロジェクト 冬のシンポジウム
• [Presentation] 記憶の維持と加齢性記憶障害におけるインスリン様シグナルによる全身性制御 (2016)
  • Author(s): 田辺憲人、伊藤素行、殿城亜矢子
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2016-12-01
• [Presentation] A critical role of insulin-like signaling in memory maintenance and age-related memory impairment in Drosophila. (2016)
  • Author(s): Ayako Tonoki, Kento Tanabe, Motoyuki Itoh.
  • Organizer: 12th Japanese Drosophila Research Conference
  • Place of Presentation: 東京
  • Year and Date: 2016-09-10
• [Presentation] ショウジョウバエにおける老化に伴う長期記憶変化とNotchシグナル (2015)
  • Author(s): 吉岡佐保、伊藤素行、殿城亜矢子
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-02
• [Presentation] 記憶の維持と加齢性記憶障害におけるインスリン様シグナルの重要な役割 (2015)
  • Author(s): 田辺憲人、伊藤素行、殿城亜矢子
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] A critical role for insulin-like signaling in memory maintenance and age-related memory impairment in Drosophila (2015)
  • Author(s): Kento Tanabe, Motoyuki Itoh, Ayako Tonoki
  • Organizer: EMBO Meeting
  • Place of Presentation: クレタ、ギリシャ
  • Year and Date: 2015-07-09
  • Int'l Joint Research
• [Presentation] Aging impairs protein synthesis dependent long-term memory in Drosophila (2014)
  • Author(s): Ayako Tonoki, Ronald L. Davis.
  • Organizer: The 11th Japanese Drosophila research conference
  • Place of Presentation: 金沢歌劇座（石川県）
  • Year and Date: 2014-06-04 – 2014-06-06
• [Remarks] 千葉大学薬学部生化学研究室ホームページ
  • URL: http://www.p.chiba-u.jp/lab/seika/index.html
• [Remarks] 生化学研究室・ホームページ
  • URL: http://www.p.chiba-u.jp/lab/seika/index.html
• [Remarks] 研究室webページ
  • URL: http://www.p.chiba-u.jp/lab/seika/index.html
• [Remarks] 千葉大学薬学研究院生化学研究室
  • URL: http://www.p.chiba-u.jp/lab/seika/index.html
• [Remarks] 千葉大学テニュアトラック制
  • URL: http://www.chiba-u.ac.jp/tenure/