Investigation of the auditory cortex as associative memory circuits

• Project/Area Number: 26830008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Niigata University
• Principal Investigator: Tsukano Hiroaki 新潟大学, 脳研究所, 助教 (90624338)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 聴覚野 / 内側膝状体腹側核 / マウス / 自然音 / フラビンイメージング / 二光子イメージング / 視床皮質路 / 和音 / 二光子 / イメージング / トノトピー / 2/3層 / 3b/4層 / 内側膝状体 / フラビン蛋白蛍光イメージング / 神経トレーシング / 記憶 / 想起 / 可塑性 / 脳機能イメージング / 大脳聴覚野

Outline of Final Research Achievements

The activity of harmony-selective neurons in the mouse auditory cortex was observed using two-photon imaging and investigated in which layer harmony-selective neurons appear. We conducted comprehensive analysis using many other natural sounds and revealed a part of the underlying principle that the auditory cortex processes natural sound. Furthermore, we tried to delineate a fine-grained mouse auditory cortex map using combination of flavoprotein fluorescence imaging and immunohistochemistry. Based on the refined map, we investigated the connective patterns with the auditory thalamus (the ventral division of the medial geniculate body), and found that the ventral division of the medial geniculate body is not single structure but is composed of multiple compartments with distinct frequency organizations.

Research Products

• [Journal Article] Tomographic optical imaging of cortical responses after crossing nerve transfer in mice (2018)
  • Author(s): Maniwa K, Yamashita H, Tsukano H, Hishida R, Endo N, Shibata M, Shibuki K
  • Journal Title: PLoS One Volume: 13 Issue: 2 Pages: e0193017-e0193017
  • DOI: 10.1371/journal.pone.0193017
  • Peer Reviewed / Open Access
• [Journal Article] Chondroitin Sulfate Is Required for Onset and Offset of Critical Period Plasticity in Visual Cortex (2017)
  • Author(s): Hou X, Yoshioka N, Tsukano H, Sakai A, Miyata S, Watanabe Y, Yanagawa Y, Sakimura K, Takeuchi K, Kitagawa H, Hensch TK, Shibuki K, Igarashi M, Sugiyama S
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 12646-12646
  • DOI: 10.1038/s41598-017-04007-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Corrigendum: Reconsidering Tonotopic Maps in the Auditory Cortex and Lemniscal Auditory Thalamus in Mice (2017)
  • Author(s): Tsukano H, Horie M, Ohga S, Takahashi K, Kubota Y, Hishida R, Takebayashi H, Shibuki K
  • Journal Title: Frontiers in Neural Circuits Volume: 11 Pages: 39-39
  • DOI: 10.3389/fncir.2017.00039
  • Peer Reviewed / Open Access
• [Journal Article] Reconsidering Tonotopic Maps in the Auditory Cortex and Lemniscal Auditory Thalamus in Mice. (2017)
  • Author(s): 1.Tsukano H, Horie M, Ohga S, Takahashi K, Kubota Y, Hishida R, Takebayashi H, Shibuki K
  • Journal Title: Front Neural Circuits Volume: 11 Pages: 14-14
  • DOI: 10.3389/fncir.2017.00014
  • Peer Reviewed / Open Access
• [Journal Article] Independent tonotopy and thalamocortical projection patterns in two adjacent parts of the classical primary auditory cortex in mice. (2017)
  • Author(s): 2.Tsukano H, Horie M, Takahashi K, Hishida R, Takebayashi H, Shibuki K
  • Journal Title: Neurosci Lett Volume: 637 Pages: 26-30
  • DOI: 10.1016/j.neulet.2016.11.062
  • Peer Reviewed / Open Access
• [Journal Article] Auditory cortical field coding long-lasting tonal offsets in mice. (2016)
  • Author(s): 3.Baba H, Tsukano H, Hishida R, Takahashi K, Horii A, Takahashi S, Shibuki K
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 34421-34421
  • DOI: 10.1038/srep34421
  • Peer Reviewed / Open Access
• [Journal Article] Quantitative map of multiple auditory cortical regions with a stereotaxic fine-scale atlas of the mouse brain. (2016)
  • Author(s): Hiroaki Tsukano, Masao Horie, Ryuichi Hishida, Kuniyuki Takahashi, Hirohide Takebayashi, Katsuei Shibuki
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 22315-22315
  • DOI: 10.1038/srep22315
  • Peer Reviewed / Open Access
• [Journal Article] Specific distribution of non-phosphorylated neurofilaments characterizing each subfield in the mouse auditory cortex. (2015)
  • Author(s): Horie M, Tsukano H, Takebayashi H, Shibuki K
  • Journal Title: Neuroscience Letters Volume: 606 Pages: 182-187
  • DOI: 10.1016/j.neulet.2015.08.055
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Spinal mechanisms underlying potentiation of hindpaw responses observed after transient hindpaw ischemia in mice. (2015)
  • Author(s): Tatsunori Watanabe, Mika Sasaki, Seiji Komagata, Hiroaki Tsukano, Ryuichi Hishida, Tatsuro Kohno, Hiroshi Baba, Katsuei Shibuki
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 11191-11191
  • DOI: 10.1038/srep11191
  • Peer Reviewed / Open Access
• [Journal Article] Delineation of a frequency-organized region isolated from the mouse primary auditory cortex. (2015)
  • Author(s): Tsukano H, Horie M, Bo T, Uchimura A, Hishida R, Kudoh M, Takahashi K, Takebayashi H, Shibuki K
  • Journal Title: J Neurophysiol Volume: 印刷中 Issue: 7 Pages: 2900-2920
  • DOI: 10.1152/jn.00932.2014
  • Peer Reviewed / Open Access
• [Journal Article] mpaired clustered protocadherin-α leads to aggregated retinogeniculate terminals and impaired visual acuity in mice. (2015)
  • Author(s): Meguro R, Hishida R, Tsukano H, Yoshitake K, Imamura R, Tohmi M, Kitsukawa T, Hirabayashi T, Yagi T, Takebayashi H, Shibuki K
  • Journal Title: J Neurochem Volume: 133 Issue: 1 Pages: 66-72
  • DOI: 10.1111/jnc.13053
  • Peer Reviewed / Open Access
• [Presentation] マウス聴覚野における二次聴覚野特異的な扁桃体との双方向結合 (2018)
  • Author(s): 塚野浩明、澁木克栄
  • Organizer: 第123回日本解剖学会総会・全国学術集会
  • Invited
• [Presentation] マウス二次聴覚野に入力する新たな視床経路の発見 (2018)
  • Author(s): 塚野浩明、大賀慎平、堀江正男、寺島裕貴、西尾奈々、竹林浩秀、澁木克栄
  • Organizer: 第7回新潟脳研－霊長研－生理研合同シンポジウム
• [Presentation] Characterization of higher order fields in the mouse auditory cortex (2018)
  • Author(s): Tsukano H, Ohga S, Horie M, Hou X, Terashima H, Kitaura H, Sugiyama S, Kakita A, Takebayashi H, Shibuki K
  • Organizer: 第8回共同研究拠点国際シンポジウムThe innovative progress of neuroscientific research through the use of advanced animal models
  • Int'l Joint Research
• [Presentation] 自然音刺激を用いたマウス聴覚野構造解析の試み (2018)
  • Author(s): 寺島裕貴、塚野浩明、古川茂人
  • Organizer: 脳と心のメカニズムMechanism of Brain and Mind
• [Presentation] Spinal NO production visualized during hindpaw ischemia and NO-induced spinal potentiation in mice (2017)
  • Author(s): Onishi T, Watanabe T, Kohno T, Tsukano H, Hishida R, Baba H, Shibuki K
  • Organizer: 第94回日本生理学会
  • Place of Presentation: アクトシティ浜松、静岡
  • Year and Date: 2017-03-28
• [Presentation] Frequency organization of the secondary auditory fields reflecting tonotopically-arranged afferents from the primary auditory thalamus (2017)
  • Author(s): Tsukano H, Shibuki K
  • Organizer: 第94回日本生理学会
  • Place of Presentation: アクトシティ浜松、静岡
  • Year and Date: 2017-03-28
• [Presentation] Higher cortical functions required for sound-shape associative learning in mice (2017)
  • Author(s): Ogi M, Yamagishi T, Tsukano H, Kamatani D, Hishida R, Horii A, Yagi T, Shibuki K
  • Organizer: 第94回日本生理学会
  • Place of Presentation: アクトシティ浜松、静岡
  • Year and Date: 2017-03-28
• [Presentation] Analysis of prediction error responses in the mouse posterior parietal cortex (2017)
  • Author(s): Yoshitake K, Tsukano H, Hishida R, Yagi T, Shibuki K
  • Organizer: 第94回日本生理学会
  • Place of Presentation: アクトシティ浜松、静岡
  • Year and Date: 2017-03-28
• [Presentation] Ventral visual cortex as a candidate for a higher area responsible for shape recognition in mice (2017)
  • Author(s): Nana N, Ogi M, Yamagishi T, Tsukano H, Hishida R, Yagi T, Shibuki K
  • Organizer: 第94回日本生理学会
  • Place of Presentation: アクトシティ浜松、静岡
  • Year and Date: 2017-03-28
• [Presentation] Spinal potentiation after hindpaw ischemia mediated by group II mGluRs and nitric oxide in mice (2017)
  • Author(s): Onishi T, Watanabe T, Sasaki M, Kamiya Y, Kohno T, Horie M, Tsukano H, Hishida R, Takebayashi H, Baba H, Shibuki K
  • Organizer: Society for Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Abnormal auditory OFF responses recorded from the cortex of an EGF-induced schizophrenia rat model (2017)
  • Author(s): Inaba H, Namba H, Tsukano H, Shibuki K, Nawa H
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] Tonotopic properties in the secondary auditory field are derived from structural organization of the ventral medial geniculate body and thalamocortical projections in mice (2017)
  • Author(s): Tsukano H, Ohga S, Horie M, Terashima H, Takebayashi H, Shibuki K
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] Plastic changes of visual responses in the primary visual cortex are induced selectively by inhibitory projection from posterior parietal cortex in mice (2017)
  • Author(s): Hishida R, Horie M, Tsukano H, Tohmi M, Shibuki K
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] Complexity of sound stimuli required for sound-shape associative responses in the mouse auditory cortex (2017)
  • Author(s): Ogi M, Yamagishi T, Tsukano H, Nishio N, Hishida R, Horii A, Yagi T, Shibuki K
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] Optical imaging of temporal cortical areas involved in audiovisual integration in awake mice (2017)
  • Author(s): Nishio N, Ogi M, Yamagishi T, Tsukano H, Hishida R, Yagi T, Shibuki K
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] Analysis of prediction error responses in the posterior parietal cortex of awake mice (2017)
  • Author(s): Yoshitake K, Tsukano H, Hishida R, Yagi T, Shibuki K
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] マウス大脳聴覚野における和音・不協和音の識別機構 (2017)
  • Author(s): 塚野浩明、澁木克栄
  • Organizer: 聴覚研究会
• [Presentation] Neuropathologic implication of pallidal hyperactivity in the auditory abnormality of schizophrenia animal models (2017)
  • Author(s): Nawa H, Sotoyama H, Narihara I, Tsukano H, Jodo E, Eifuku S, Namba H
  • Organizer: 13th World Congress of Biological Psychiatry
  • Int'l Joint Research / Invited
• [Presentation] Functional properties in the secondary auditory field are derived from structural organization of primary auditory thalamus and thalamocortical projections in mice (2016)
  • Author(s): Tsukano H, Shibuki K
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-11-11
  • Int'l Joint Research
• [Presentation] Functional footprints of impaired consciousness in mice with reduced molecular diversity of clustered protocadherin-α (2016)
  • Author(s): Shibuki K, Yamagishi T, Kamatani D, Yoshitake K, Tsukano H, Watanabe K, Hishida R, Takahashi K, Takahashi S, Horii A, Yagi T
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-11-11
  • Int'l Joint Research
• [Presentation] Prediction error responses in the mouse posterior parietal cortex are dependent on molecular diversity of clustered protocadherin α (2016)
  • Author(s): Yoshitake K, Tsukano H, Hishida R, Yagi T, Shibuki K
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-11-11
  • Int'l Joint Research
• [Presentation] Visualized spinal NO production after ischemic treatment applied to the hindpaw and NO-induced spinal potentiation in mice (2016)
  • Author(s): Onishi T, Watanabe T, Tsukano H, Hishida R, Kohno T, Baba H, Shibuki K
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2016-07-20
• [Presentation] Formation of voice-dependent associative memory circuits in primary auditory cortex in mice (2016)
  • Author(s): Tsukano H, Shibuki K
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2016-07-20
• [Presentation] Inhibition derived from parietal association area regulates the neural properties of the primary visual cortex in mice (2016)
  • Author(s): Hishida R, Horie M, Tsukano H, Tohmi M, Shibuki K
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2016-07-20
• [Presentation] Higher cortical functions required for sound-shape associative learning in mice (2016)
  • Author(s): Ogi M, Yamagishi T, Tsukano H, Kamatani D, Hishida R, Horii A, Yagi T, Shibuki K
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2016-07-20
• [Presentation] Impaired prediction error responses in the posterior parietal cortex of mice with reduced diversity of protocadherin-α (2016)
  • Author(s): Yoshitake K, Tsukano H, Hishida R, Yagi T, Shibuki K
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2016-07-20
• [Presentation] Hyperdopaminergic activity and abnormal auditory processing in a cytokine-induced schizophrenia model (2016)
  • Author(s): Nawa H, Sotoyama H, Tsukano H, Shibuki K, Namba H
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2016-07-20
• [Presentation] Voice dependent formation of associative memory circuit in mouse primary auditory cortex (2016)
  • Author(s): Tsukano H, Shibuki K
  • Organizer: 第93回日本生理学会
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Bilateral spinal potentiation induced by NO after hemilateral and transient ischemia applied to the mouse hindpaw (2016)
  • Author(s): Onishi T, Watanabe T, Tsukano H, Hishida R, Kohno T, Baba H, Shibuki K
  • Organizer: 第93回日本生理学会
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Neuroinflammatory features of the cytokine-induced animal model for schizophrenia; implication of the regional specificity (2015)
  • Author(s): Nawa H, Tsukano H, Shibuki K, Kakita A, Sotoyama H, Namba H
  • Organizer: 第58回日本神経化学会大会
  • Place of Presentation: 大宮ソニックシティ（埼玉県、さいたま市）
  • Year and Date: 2015-09-11
• [Presentation] Newly identified region in the auditory cortex responding to ultrasonic male songs in mice (2015)
  • Author(s): Tsukano H, Hishida R, Shibuki K
  • Organizer: 第38回日本神経科学学会
  • Place of Presentation: 神戸国際会議場（兵庫県、神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Spinal potentiation contralateral to the ischemic treatment applied to the hindpaw is mediated by nitric oxide (2015)
  • Author(s): Onishi T, Watanabe T, Tsukano H, Hishida R, Kohno T, Baba H, Shibuki K
  • Organizer: 第38回日本神経科学学会
  • Place of Presentation: 神戸国際会議場（兵庫県、神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Prediction error responses in the mouse posterior parietal cortex are produced by experience (2015)
  • Author(s): Yoshitake K, Tsukano H, Hishida R, Yagi T, Shibuki K
  • Organizer: 第38回日本神経科学学会
  • Place of Presentation: 神戸国際会議場（兵庫県、神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Two-photon imaging analysis of inhibitory projections from the parietal association area to the primary visual cortex in mice (2015)
  • Author(s): Hishida R, Horie M, Tsukano H, Tohmi M, Shibuki K
  • Organizer: 第38回日本神経科学学会
  • Place of Presentation: 神戸国際会議場（兵庫県、神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Re-definition of the primary auditory cortex by separating a newly identified region and their functional specialization in mice (2015)
  • Author(s): Tsukano H, Shibuki K
  • Organizer: 第92回日本生理学会
  • Place of Presentation: 神戸国際会議場（兵庫）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Ischemia-induced potentiation of cortical responses to hindpaw stimulation is partly mediated by nitric oxide at the spinal cord level (2015)
  • Author(s): Ohnishi T, Watanabe T, Tsukano H, Hishida R, Kohno T, Baba H, Shibuki K
  • Organizer: 第92回日本生理学会
  • Place of Presentation: 神戸国際会議場（兵庫）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] The secondary auditory cortex receives topological projections from the ventral division of the medial geniculate body in mice (2015)
  • Author(s): Ohga S, Tsukano H, Shibuki K
  • Organizer: 第92回日本生理学会
  • Place of Presentation: 神戸国際会議場（兵庫）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Prediction error responses in the mouse posterior parietal cortex are dependent on protocadherin-α diversity (2015)
  • Author(s): Yoshitake K, Tsukano H, Hishida R, Yagi T, Shibuki K
  • Organizer: 第92回日本生理学会
  • Place of Presentation: 神戸国際会議場（兵庫）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Higher visual cortices responsible for shape recognition in mice (2015)
  • Author(s): Yamagishi T, Tsukano H, Kamatani D, Hishida R, Yamamoto Y, Yagi T, Shibuki K
  • Organizer: 第92回日本生理学会
  • Place of Presentation: 神戸国際会議場（兵庫）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Postnatal developmental observations of abnormal retinal terminal aggregation in the cPcdh- α KO mice (2015)
  • Author(s): Meguro R, Hishida R, Tsukano H, Yoshitake K, Kitsukawa T, Hirabayashi K, Takebayashi H, Yagi T, Shibuki K
  • Organizer: 第92回日本生理学会
  • Place of Presentation: 神戸国際会議場（兵庫）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Somatosensory Cortical Responses after Crossing Nerve Transfer in Mice (2015)
  • Author(s): Maniwa K, Yamashita H, Tsukano H, Hishida R, Endo N, Shibata M, Shibuki K
  • Organizer: 第92回日本生理学会
  • Place of Presentation: 神戸国際会議場（兵庫）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Transcranial imaging of cortical activities after sound-shape association in mice (2014)
  • Author(s): Tatsuya Yamagishi, Hiroaki Tsukano, Daiki Kamatani, Ryuichi Hishida, Sugata Takahashi, Katsuei Shibuki
  • Organizer: 第37回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Inhibitory controls from the parietal association area to the primary visual cortex in mice (2014)
  • Author(s): Ryuichi Hishida, Masao Horie, Hiroaki Tsukano, Manavu Tohmi, Katsuei Shibuki
  • Organizer: 第37回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Dorsal high frequency area of the primary auditory cortex responding to ultrasonic male songs in mice (2014)
  • Author(s): Hiroaki Tsukano, Masao Horie, Ryuichi Hishida, Katsuei Shibuki
  • Organizer: 第37回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Impaired multisensory responses in the posterior parietal cortex of mice with a reduced cluster number of protocadherin-α (2014)
  • Author(s): Kohei Yoshitake, Hiroaki Tsukano, Manabu Tohmi, Ryuichi Hishida, Takeshi Yagi, Katsuei Shibuki
  • Organizer: 第37回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Roles of the extrageniculate pathway in properties of higher visual cortices of mice (2014)
  • Author(s): Manavu Tohmi, Reiko Meguro, Hiroaki Tsukano, Ryuichi Hishida, Masao Norita, Katsuei Shibuki
  • Organizer: 第37回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Direct and indirect somatosensory cortical responses after crossing nerve transfer in mice (2014)
  • Author(s): Keiichi Maniwa, Haruyoshi Yamashita, Hiroaki Tsukano, Ryuichi Hishida, Minoru Shibata, Naoto Endo, Katsuei Shibuki
  • Organizer: 第37回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Remarks] 研究室ホームページ
  • URL: http://www.bri.niigata-u.ac.jp/~physio/
• [Remarks] 個人ホームページ
  • URL: http://1qaz2wsx3edc.webcrow.jp/TsukanoH.html
• [Remarks] 光学イメージングを用いて見えてきた、新しいマウス大脳聴覚野マップ
  • URL: http://www.bri.niigata-u.ac.jp/column/000573.html