In vivo mechanisms of dendritic development

• Project/Area Number: 26830017
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Keio University
• Principal Investigator: Takeo Yukari 慶應義塾大学, 医学部, 研究員 (90624320)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 樹状突起 / イメージング / 神経活動 / In vivoイメージング / in vivoイメージング / プルキンエ細胞

Outline of Final Research Achievements

Neurons develop cell-type specific dendritic morphology which is thought to be important for normal circuit development and functions. This study aimed to reveal the in vivo mechanisms for dendritic development. I focused on the cerebellar Purkinje cells, which has unique and elaborate dendritic morphology. First, I revealed that RORα, a transcriptional factor, is crucial for both development and maintenance of dendrite morphology (J Neurosci, 2015). Next, using in vivo two-photon imaging method, I revealed that multiple dendrites actively change their morphology before pruning. Furthermore,this process requires neural activities and intracellular calcium signaling.These results contribute to understanding of the mechanisms how neural cuicuit develops in vivo.

Research Products

• [Journal Article] A novel non-canonical Notch signaling regulates expression of synaptic vesicle proteins in excitatory neurons (2016)
  • Author(s): Hayashi Y, Nishimune H, Hozumi K, Saga Y, Harada A, Yuzaki M, Iwatsubo T, Kopan R, Tomita T.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 23969-23969
  • DOI: 10.1038/srep23969
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] RORalpha regulates multiple aspects of dendrite development in cerebellar Purkinje cells in vivo. (2015)
  • Author(s): Takeo, Y.H., Kakegawa, W., Miura, E., Yuzaki, M.
  • Journal Title: The Journal of Neuroscience Volume: 35 Issue: 36 Pages: 12518-12534
  • DOI: 10.1523/jneurosci.0075-15.2015
  • Peer Reviewed
• [Journal Article] Purkinje細胞の樹状突起形成機構―新しい方法で古い転写因子RORαの役割解明に挑む (2015)
  • Author(s): 竹尾 ゆかり、柚﨑 通介
  • Journal Title: 医学のあゆみ Volume: 255 Pages: 940-946
• [Journal Article] Anterograde C1ql1 signaling is required in order to determine and maintain a single-winner climbing fiber in the mouse cerebellum. (2015)
  • Author(s): Kakegawa W, Mitakidis N, Miura E, Abe M, Matsuda K, Takeo YH, Kohda K, Motohashi J, Takahashi A, Nagao S, Muramatsu SI, Watanabe M, Sakimura K, Aricescu AR, Yuzaki M
  • Journal Title: Neuron Volume: 85 Issue: 2 Pages: 316-329
  • DOI: 10.1016/j.neuron.2014.12.020
  • Peer Reviewed / Open Access
• [Presentation] Multiple roles of RORα in dendritic development of cerebellar Purkinje cells in vivo (2015)
  • Author(s): Yukari H. Takeo
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場(兵庫県神戸市)
  • Year and Date: 2015-07-28
  • Int'l Joint Research
• [Presentation] Optogenetic control of the function of the Golgi apparatus in neurons (2014)
  • Author(s): Junnosuke Endo, Yukari Takeo, Shinji Matsuda and Michisuke Yuzaki
  • Organizer: 2014 Joint Meeting of the Society for Glycobiology and the Japanese Society of Carbohydrate Research
  • Place of Presentation: ホノルル(米国)
  • Year and Date: 2014-11-17 – 2014-11-21
• [Presentation] Optogenetic control of the function of the Golgi apparatus in neurons (2014)
  • Author(s): Junnosuke Endo, Yukari Takeo, Shinji Matsuda and Michisuke Yuzaki
  • Organizer: 第8回神経糖鎖生物学領域班会議“Glycans in Neuroscience” (Satellite Symposium II)
  • Place of Presentation: ホノルル(米国)
  • Year and Date: 2014-11-16
• [Presentation] Stage-specific roles for ROR in morphogenesis and maintenance (2014)
  • Author(s): 竹尾 ゆかり
  • Organizer: 第48回慶應ニューロサイエンス学会
  • Place of Presentation: 慶應、信濃町（東京）
  • Year and Date: 2014-08-09