Revelation of the induction mechanism of plasticity during motor learning with the two-photon imaging and optical manipulation

• Project/Area Number: 26830020
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: The University of Tokyo (2016); National Institute for Basic Biology (2014-2015)
• Principal Investigator: MASAMIZU Yoshito 東京大学, 大学院医学系研究科(医学部), 助教 (90608530)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ２光子イメージング / 2光子励起顕微鏡 / 運動学習 / アデノ随伴ウイルス / 逆行性感染 / 2光子カルシウムイメージング

Outline of Final Research Achievements

First, fluorescence calcium sensor was expressed in only corticostriatal neurons or corticospinal neurons of mouse primary motor cortex by the retrograde gene transfer of adeno-associated virus. Next, I performed two-photon calcium imaging during a self-initiated lever-pull task for two weeks. These imaging data revealed that, during motor learning, the population which increased firing rate in corticostriatal neurons was different from that in corticospinal neurons.

Research Products

• [Journal Article] Two distinct layer-specific dynamics of cortical ensembles during learning of a motor task. (2014)
  • Author(s): Masamizu Y, Tanaka YR, Tanaka YH, Hira R, Ohkubo F, Kitamura K, Isomura Y, Okada T, Matsuzaki M.
  • Journal Title: Nat. Neurosci Volume: 17 Issue: 7 Pages: 987-994
  • DOI: 10.1038/nn.3739
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Reward-timing-dependent bidirectional modulation of cortical microcircuits during optical single-neuron operant conditioning. (2014)
  • Author(s): Hira R et al.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 5551-5551
  • DOI: 10.1038/ncomms6551
  • Peer Reviewed / Open Access
• [Presentation] 運動課題学習中の一次運動野第2/3および第5a層での集団および個々の細胞における神経活動変化 (2014)
  • Author(s): 正水 芳人
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜, 横浜, 神奈川県
  • Year and Date: 2014-09-11
• [Remarks] 運動学習は大脳皮質深部の神経細胞活動パターンとして記憶される
  • URL: http://www.nibb.ac.jp/press/2014/06/02.html