Reward dependent synaptic plasticity during single neuron operant conditioning

• Project/Area Number: 26830021
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Hira Riichiro 基礎生物学研究所, 光脳回路研究部門, 助教 (80712299)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: ブレインマシーンインターフェース / ２光子イメージング / 光遺伝学 / 2光子イメージング / 運動野 / BMI / オプトジェネティクス / 随意制御

Outline of Final Research Achievements

In this study, we developed several new methods including a real time analysis system of images obtained by two photon calcium imaging of cortical neurons and an experimental system that allowed us to simultaneously conduct two photon imaging and the optogenetic stimulation. We found that the reward timing dependent bidirectional modulation (RTBM) of cortical neurons during the learning of the single neuron operant conditioning. The RTBM might be one of the key mechanism that enables animal to learn to use brain machine interface.

Research Products

• [Journal Article] Distinct functional modules for discrete and rhythmic forelimb movements in the mouse motor cortex. (2015)
  • Author(s): Hira R., Terada S., Kondo M., Matsuzaki M.
  • Journal Title: Journal of Neuroscience Volume: 35 Issue: 39 Pages: 13311-13322
  • DOI: 10.1523/jneurosci.2731-15.2015
  • Peer Reviewed
• [Journal Article] Reward-timing-dependent bidirectional modulation of cortical microcircuits during optical single-neuron operant conditioning. (2014)
  • Author(s): Hira R et al.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 5551-5551
  • DOI: 10.1038/ncomms6551
  • Peer Reviewed / Open Access
• [Presentation] Optogenetic mapping of cerebro-cerebellar communication loop in mice (2016)
  • Author(s): Choo M, Hira R, Mastuzaki M, Kano M, Kitamura K.
  • Organizer: Physiology Society of Japan
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌）
  • Year and Date: 2016-03-22
• [Presentation] Distinct thalamocortical activities in the motor cortex during voluntary movements (2015)
  • Author(s): Y. H. Tanaka, Y. R. Tanaka, R. Hira, M. Kondo, S. Terada, Y. Kawaguchi, M. Matsuzaki.
  • Organizer: Society of Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Single-neuron operant conditioning by two-photon imaging induces reward-timing-dependent modulation in cortical microcircuit (2014)
  • Author(s): R. Hira, F. Ohkubo, Y. Masamizu, M. Ohkura, J. Nakai, T. Okada, M. Matsuzaki
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 神奈川県横浜市 パシフィコ横浜
  • Year and Date: 2014-09-12
• [Presentation] SINGLE-NEURON OPERANT CONDITIONING BY TWO-PHOTON IMAGING INDUCES REWARD-TIMING-DEPENDENT BIDIRECTIONAL MODULATIONS IN CORTICAL MICROCIRCUIT (2014)
  • Author(s): R. Hira, F. Ohkubo, Y. Masamizu, M. Ohkura, J. Nakai, T. Okada, M. Matsuzaki,
  • Organizer: FENS (Federation of European Neurosciences)
  • Place of Presentation: イタリア ローマ Milano Congressi
  • Year and Date: 2014-06-06
• [Book] 科学と文化をつなぐアナロジーという思考様式 (2016)
  • Author(s): 平理一郎 春日直樹(編)
  • Publisher: 東京大学出版会