Analysis of biochemical computation and dynamics in neurons.
Project/Area Number |
26830043
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | The University of Tokyo |
Principal Investigator |
Fujii Hajime 東京大学, 大学院医学系研究科(医学部), 助教 (80717546)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | Ca2+シグナル / シナプス可塑性 / シナプス / シグナル伝達 / 神経科学 / 生化学 / イメージング / Ca2+シグナリング / 蛍光プローブ / 顕微鏡 / 分子生物学 |
Outline of Final Research Achievements |
Understanding how complex neuronal activities and biochemical computation induced by synaptic input in neuronal circuit is fundamental question in neuroscience. To begin to answer that question, direct measurement of biochemical activities in synapses of living neuron is necessary. We developed new probes for measurement of Ca2+ dependent kinase (CaMKII) and Ca2+ dependent phosphatase (calcineurin) activities, which have improved signal to noise ratio. With these new probes, we investigated Ca2+ dependent kinase and phosphatase activities in dendrites and synapses of living neurons during induction phase of synaptic plasticity. Distinct spatial and temporal profiles between the two enzymes was discovered. These results provide new insights into the biochemical computation in neurons and shed new light on how the brain works.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Rational design of a high-affinity, fast, red calcium indicator R-CaMP2.2015
Author(s)
Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
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Journal Title
Nat Methods
Volume: 12
Issue: 1
Pages: 64-70
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] カルシウムシグナリングと脳高次機能制御2016
Author(s)
金亮, 井上昌俊, 野中美応, 川島尚之, 奧野浩行, 竹本-木村さやか, 坂本雅行, 藤井哉, 尾藤晴彦
Organizer
私立大学戦略的研究基盤形成支援事業 中間報告会シンポジウム「次世代の包括的緩和医療を支える最先端基礎/臨床融合型研究の基盤形成」
Place of Presentation
東京
Related Report
Invited
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[Presentation] Rational design of ultrafast, high-affinity calcium indicators for monitoring neuronal activity2016
Author(s)
Inoue M, Takeuchi A, HoriganeS, Fujii H, Kamijo S, Takemoto-Kimura S, Ohkura M, Gengyo-Ando K, Kano M, Nakai J, Kitamura K, Bito H.
Organizer
The 7th International Neural Microcircuit Conference
Place of Presentation
Okazaki
Related Report
Int'l Joint Research / Invited
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[Presentation] Rational design of ultrafast, high-affinity red calcium indicator for monitoring neuronal activity.2015
Author(s)
Inoue M, Takeuchi A, Horigane S, Fujii H, Kamijo S, Takemoto-Kimura S, Ohkura M, Gengyo-Ando K, Kano M, Nakai J, Kitamura K, Bito H
Organizer
The 45th Annual Meeting of the Society for Neuroscience
Place of Presentation
Chicago, USA
Year and Date
2015-10-18
Related Report
Int'l Joint Research
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[Presentation] Rational design of a novel high-affinity, ultrafast, red calcium indicator R-CaMP22015
Author(s)
Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
Organizer
第92回日本生理学会大会
Place of Presentation
神戸
Year and Date
2015-03-21
Related Report
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[Presentation] Rational design of a high-affinity, fast, red calcium indicator R-CaMP22015
Author(s)
Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
Organizer
第88回日本薬理学会年会
Place of Presentation
名古屋
Year and Date
2015-03-18
Related Report
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[Presentation] Rational design of a novel high-affinity, ultrafast, red calcium indicator R-CaMP22014
Author(s)
Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
Organizer
2014年度包括脳ネットワーク冬のシンポジウム
Place of Presentation
東京
Year and Date
2014-12-11
Related Report
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[Presentation] Rational design of a high-affinity, fast, red calcium indicator R-CaMP22014
Author(s)
Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
Organizer
生理学研究所 研究会「シナプス・神経ネットワークの機能ダイナミクス」
Place of Presentation
岡崎
Year and Date
2014-12-02
Related Report
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[Presentation] Signaling from synapse to the nucleus and imaging of active neuronal ensembles2014
Author(s)
Bito H, Inoue M, Nonaka M, Yagishita-Kyo N, Kawashima T, Suzuki K, Ishii Y, Fujii H, Endo T, Goto M, Koyama H, Takemoto-Kimura S, Kim R
Organizer
第37回日本神経科学大会
Place of Presentation
横浜
Year and Date
2014-09-11
Related Report
Invited