Regulatory mechanisms underlying neuronal polarization
| Project/Area Number |
26830045
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Nagoya University |
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Principal Investigator |
Tetsuya Takano 名古屋大学, 医学(系)研究科(研究院), 研究員 (00725541)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | Neuronal polarity / RhoA / Rho-kinase / CaMKI / 神経極性 / 発生 / 細胞極性 / 軸索形成 / 樹状突起形成 |
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| Outline of Final Research Achievements |
Neurons are highly polarized cells, which have two structurally and functionally distinct compartments, ie., axons and dendrites. Neuronal polarization is precisely established by local activation signal and long-range inhibitory signaling (Takano et al., 2015, Development). However, the long-range inhibitory signaling that prevents multiple axonal formation remains largely unknown.
In this year, we found that local application of NT-3 to axon induced a long-range Ca2+ propagation that led to activate RhoA in the cell body through CaMKI. The local inhibition of Rho-kinase in the minor neurites induced neurite elongation and subsequently formed multiple axons. We performed phospho-proteomic analysis (the KISS method) to identify novel substrates for CaMKI. We found that CaMKI phosphorylated a RhoGEF, and enhanced its GEF activity. Together, long-range signaling of Ca2+/CaMKI/RhoGEF/RhoA/Rho-kinase might be a novel inhibitory pathway responsible for neuronal polarization.
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Report
(3 results)
Research Products
(11 results)
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[Presentation] Long‐range Ca2+ signaling regulates neuronal polarization via Rho‐kinase in a CaMKI‐dependent manner2016
Author(s)
Tetsuya Takano, Mengya Wu, Naruki Ishizawa, Shinichi Nakamuta, Naoki Honda, Takashi Namba, Takashi Watanabe, Chundi Xu, Tomonari Hamaguchi, Yoshimitsu Yura, Mutsuki Amano, Shin Ishii, Klaus M. Hahn and Kozo Kaibuchi
Organizer
第3回国際シンポジウム Neocortical organization
Place of Presentation
東京大学小柴ホール、東京都文京区
Year and Date
2016-02-11
Related Report
Int'l Joint Research
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[Presentation] Rho-kinaseによる神経細胞の極性形成・維持機構の解明2014
Author(s)
Tetsuya Takano, Shinichi Nakamuta, Chundi Xu, Takashi Namba, Mutsuki Amano and Kozo Kaibuchi
Organizer
第57回日本神経化学会大会
Place of Presentation
奈良県文化会館、奈良県新公会堂 (奈良県奈良市）
Year and Date
2014-09-29 – 2014-10-01
Related Report
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