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Regulatory mechanisms underlying neuronal polarization

Research Project

Project/Area Number 26830045
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNagoya University

Principal Investigator

Tetsuya Takano  名古屋大学, 医学(系)研究科(研究院), 研究員 (00725541)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
KeywordsNeuronal polarity / RhoA / Rho-kinase / CaMKI / 神経極性 / 発生 / 細胞極性 / 軸索形成 / 樹状突起形成
Outline of Final Research Achievements

Neurons are highly polarized cells, which have two structurally and functionally distinct compartments, ie., axons and dendrites. Neuronal polarization is precisely established by local activation signal and long-range inhibitory signaling (Takano et al., 2015, Development). However, the long-range inhibitory signaling that prevents multiple axonal formation remains largely unknown.
In this year, we found that local application of NT-3 to axon induced a long-range Ca2+ propagation that led to activate RhoA in the cell body through CaMKI. The local inhibition of Rho-kinase in the minor neurites induced neurite elongation and subsequently formed multiple axons. We performed phospho-proteomic analysis (the KISS method) to identify novel substrates for CaMKI. We found that CaMKI phosphorylated a RhoGEF, and enhanced its GEF activity. Together, long-range signaling of Ca2+/CaMKI/RhoGEF/RhoA/Rho-kinase might be a novel inhibitory pathway responsible for neuronal polarization.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014 Other

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results) Remarks (2 results)

  • [Journal Article] Extracellular and Intracellular Signaling for Neuronal Polarity.2015

    • Author(s)
      Namba, T.;Funahashi, Y.; Nakamuta, S.;Xu, C.;Takano, T.;Kaibuchi, K.
    • Journal Title

      Physiol Rev

      Volume: 95 Issue: 3 Pages: 995-1024

    • DOI

      10.1152/physrev.00025.2014

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Extracellular and intracellular signaling for neuronal polarity2016

    • Author(s)
      貝淵 弘三、Xu Chundi1、高野 哲也
    • Organizer
      第3回国際シンポジウム Neocortical organization
    • Place of Presentation
      東京大学小柴ホール、東京都文京区
    • Year and Date
      2016-02-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Long‐range Ca2+ signaling regulates neuronal polarization via Rho‐kinase in a CaMKI‐dependent manner2016

    • Author(s)
      Tetsuya Takano, Mengya Wu, Naruki Ishizawa, Shinichi Nakamuta, Naoki Honda, Takashi Namba, Takashi Watanabe, Chundi Xu, Tomonari Hamaguchi, Yoshimitsu Yura, Mutsuki Amano, Shin Ishii, Klaus M. Hahn and Kozo Kaibuchi
    • Organizer
      第3回国際シンポジウム Neocortical organization
    • Place of Presentation
      東京大学小柴ホール、東京都文京区
    • Year and Date
      2016-02-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Long‐range Ca2+ signaling regulates neuronal polarization via Rho‐kinase in a CaMKI‐dependent manner2015

    • Author(s)
      Ishizawa, Takano, Wu, Nakamuta, Xu, Namba, Amano, Kaibuchi
    • Organizer
      ASCB 2015
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-12-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activation of RhoA/Rho-kinase by CaMKI-mediated phosphorylation of GEF-H1 regulates neuronal polarization2015

    • Author(s)
      Takano Tetsuya, Nakamuta Shinichi, Wu Mengya, Ishizawa Naruki, Xu Chundi, Namba Takashi, Amano Mutsuki, Kaibuchi Kozo
    • Organizer
      第58回日本神経化学大会
    • Place of Presentation
      大宮ソニックシティー、埼玉県大宮市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Long-range Ca2+ signaling regulates neuronal polarization via Rho-kinase in a CaMKI-dependent manner2015

    • Author(s)
      髙野 哲也, 中牟田 信一, 石澤 成樹, Chundi Xu,  難波 隆志, 天野 睦紀, 貝淵 弘三
    • Organizer
      第38回日本神経科学会
    • Place of Presentation
      神戸国際会議場、コンベンションセンター、兵庫県神戸市
    • Year and Date
      2015-07-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] Long-range Ca2+ signaling regulates neuronal polarization via Rho-kinase in a CaMKI-dependent manner2015

    • Author(s)
      Tetsuya Takano, Shinichi Nakamuta, Mengya Wu, Naruki Ishizawa, Chundi Xu, Takashi Namba, Mutsuki Amano and Kozo Kaibuchi
    • Organizer
      Developmental Neutobiology Course 2015
    • Place of Presentation
      OIST, 沖縄県国頭郡恩納村
    • Year and Date
      2015-07-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] Long-range Ca2+ signaling regulates neuronal polarization via Rho-kinase in a CaMKI-dependent manner2015

    • Author(s)
      Tetsuya Takano, Shinichi Nakamuta, Chundi Xu, Takashi Namba, Mutsuki Amano and Kozo Kaibuchi
    • Organizer
      Emerging concepts of the neuronal cytoskeleton (w15-46)
    • Place of Presentation
      Puerto Varas, Chile
    • Year and Date
      2015-03-22 – 2015-03-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Rho-kinaseによる神経細胞の極性形成・維持機構の解明2014

    • Author(s)
      Tetsuya Takano, Shinichi Nakamuta, Chundi Xu, Takashi Namba, Mutsuki Amano and Kozo Kaibuchi
    • Organizer
      第57回日本神経化学会大会
    • Place of Presentation
      奈良県文化会館、奈良県新公会堂 (奈良県奈良市)
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Research-status Report
  • [Remarks] Dept of Cell Pharmacology

    • URL

      http://www.med.nagoya-u.ac.jp/Yakuri/index.htm

    • Related Report
      2015 Annual Research Report
  • [Remarks] Dept of Cell Pharmacology

    • URL

      http://www.med.nagoya-u.ac.jp/Yakuri/

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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