| Project/Area Number |
26830059
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 軟骨形成 / 繋留因子 / 小胞輸送 / ゴルジ体 / 実験動物 / 細胞外基質 / Giantin / 軟骨基質 / 細胞内輸送 / レクチカンファミリー / 軟骨細胞 |
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| Outline of Final Research Achievements |
Giantin is vesicle tethering factor that functions at cis-Golgi compartment. Immunohistological analysis showed that giantin were expressed in various types of cells including chondrocyte. Morphology of golgi apparatus in chondrocytes lacking giantin were indistinguishable from that of normal cell by immunofluorescence of golgi marker proteins. Cartilage of embryos lacking giantin showed similar level of type II collagen and fibronectin. has markedly reduced aggrecan and link protein, and increased type XI collagen. These results suggest that tethering factors have unique and redundant roles, and that giantin play a pivotal role in secretion and/or synthesis of specific cartilage matrix.
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