• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the spatio-temporal angiogenic regulation by CUL3

Research Project

Project/Area Number 26830077
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionEhime University

Principal Investigator

Sakaue Tomohisa  愛媛大学, 医学(系)研究科(研究院), 助教(特定教員) (20709266)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsCUL3 / KCTD / 血管新生 / 血管内皮細胞 / 細胞運動 / ユビキチン
Outline of Final Research Achievements

We first identified KCTD protein as a novel angiogenic regulator by siRNA-based screening and AlphaScreening assay system. This molecule is one of the CUL3-binding protein. VEGF-A-induced angiogenic sprouting was strongly inhibited due to disorder of cell movement when KCTD was knocked down. Silencing KCTD completely phenocopied the effects of CUL3 knockdown in HUVECs. We believe that our data contribute to the understanding of the precise mechanism by which the CUL3-KCTD axis regulates angiogenesis in humans and provide insights that may help to establish a new strategy for the treatment of angiogenesis-associated diseases.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (18 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Presentation (14 results) (of which Int'l Joint Research: 1 results) Remarks (3 results)

  • [Int'l Joint Research] 米国国立衛生研究所(米国)

    • Related Report
      2015 Annual Research Report
  • [Presentation] 血管内皮細胞においてCUL3はVEGFR2の発現を制御する2016

    • Author(s)
      坂上倫久
    • Organizer
      がん支援 冬期公開シンポジウム
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2016-02-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 血管内皮細胞におけるCUL3の機能解析2015

    • Author(s)
      坂上 倫久, 藤崎 亜耶子, 中城 公一, 浜川 裕之, 泉谷 裕則, 東山 繁樹
    • Organizer
      第23回日本血管生物医学会学術集会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Analysis of novel Nrf2-binding BTB proteins using AlphaScreen assay2015

    • Author(s)
      宇都宮 果歩, 上杉 恭広, 深江 舜也, 藤崎 亜耶子, 坂上 倫久, 高橋 宏隆, 澤崎 達也, 東山 繁樹
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] KLHL20, CUL3-binding protein, regulates expression of EGFR family proteins2015

    • Author(s)
      深江 舜也, 上杉 恭広, 宇都宮 果歩, 坂上 倫久, 泉谷 裕則, 東山 繁樹.
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] CUL3 regulates expression of EGF receptor family proteins2015

    • Author(s)
      上杉 恭広, 宇都宮 果歩, 深江 舜也, 坂上 倫久, 中山 寛尚, 福田 信治, 泉谷 裕則, 東山 繁樹
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] CUL3 regulates vascular endothelial cell-specific gene expression2015

    • Author(s)
      坂上 倫久, 藤崎 亜耶子, 中城 公一, 浜川 裕之, 泉谷 裕則, 東山 繁樹
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会,
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] AlphaScreenを用いたCUL3複合体解析システムの構築2015

    • Author(s)
      藤崎 亜耶子, 宇都宮 果歩, 上杉 恭広, 深江 舜也, 坂上 倫久, 高橋 宏隆, 澤崎 達也, 東山 繁樹
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Cullin3-KCTD10 regulates angiogenesis through controlling levels of RhoA2015

    • Author(s)
      Tomohisa Sakaue, Ayako Fujisaki, Shigeki Higashiyama
    • Organizer
      Protein Island Matsuyama International Symposium 2015
    • Place of Presentation
      愛媛県松山市
    • Year and Date
      2015-08-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CUL3/SPOP/Daxx複合体によるVEGFR2の発現制御機構2015

    • Author(s)
      坂上 倫久、藤崎 亜耶子、東山 繁樹
    • Organizer
      第56回 日本生化学会 中国・四国支部例会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] BTB proteins as an adaptor for Cul3-based ubiquitin ligases multiply regulate angiogenesis2015

    • Author(s)
      Tomohisa Sakaue, Shigeki Higashiyama
    • Organizer
      Joint International Symposium on TGF-β Family and Cancer Signal Network in Tumor Microenvironment
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2015-01-12 – 2015-01-13
    • Related Report
      2014 Research-status Report
  • [Presentation] CUL3はRhoタンパク質の発現および活性制御 を介して血管新生を調節する2014

    • Author(s)
      坂上 倫久, 東山繁樹
    • Organizer
      第87回日本生化学大会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] E3ユビキチンリガーゼCUL3による血管新生制御機構の解析2014

    • Author(s)
      坂上 倫久, 東山繁樹
    • Organizer
      第19回日本病態プロテアーゼ学会学術集
    • Place of Presentation
      大阪府豊中市
    • Year and Date
      2014-08-08 – 2014-08-09
    • Related Report
      2014 Research-status Report
  • [Presentation] CUL3は多様な血管新生制御遺伝子の発現2014

    • Author(s)
      坂上 倫久, 東山繁樹
    • Organizer
      第55回日本生化学会中国四国支部例会
    • Place of Presentation
      愛媛県松山市
    • Year and Date
      2014-06-06 – 2014-06-07
    • Related Report
      2014 Research-status Report
  • [Presentation] Versatility of E3 ubiquitin ligase Cullin3 as an angiogenic balance regulator2014

    • Author(s)
      Tomohisa Sakaue, Shigeki Higashiyama
    • Organizer
      The 18th International Vascular Biology Meeting
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-04-14 – 2014-04-17
    • Related Report
      2014 Research-status Report
  • [Remarks] 愛媛大学プロテオサイエンスセンター 細胞増殖・腫瘍制御部門

    • URL

      http://www.m.ehime-u.ac.jp/school/biochem2/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 愛媛大学プロテオサイエンスセンター

    • URL

      http://www.pros.ehime-u.ac.jp/section/06.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 愛媛大学プロテオサイエンスセンター細胞増殖・腫瘍制御部門

    • URL

      http://www.m.ehime-u.ac.jp/school/biochem2/

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi