Analysis of the spatio-temporal angiogenic regulation by CUL3
| Project/Area Number |
26830077
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Ehime University |
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Principal Investigator |
Sakaue Tomohisa 愛媛大学, 医学(系)研究科(研究院), 助教(特定教員) (20709266)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | CUL3 / KCTD / 血管新生 / 血管内皮細胞 / 細胞運動 / ユビキチン |
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| Outline of Final Research Achievements |
We first identified KCTD protein as a novel angiogenic regulator by siRNA-based screening and AlphaScreening assay system. This molecule is one of the CUL3-binding protein. VEGF-A-induced angiogenic sprouting was strongly inhibited due to disorder of cell movement when KCTD was knocked down. Silencing KCTD completely phenocopied the effects of CUL3 knockdown in HUVECs. We believe that our data contribute to the understanding of the precise mechanism by which the CUL3-KCTD axis regulates angiogenesis in humans and provide insights that may help to establish a new strategy for the treatment of angiogenesis-associated diseases.
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Report
(3 results)
Research Products
(18 results)
