| Project/Area Number |
26830088
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Masatoshi Ohgushi 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 上級研究員 (00462664)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ヒトES細胞 / 染色体安定性 / Rhoシグナル経路 / 多能性幹細胞 / ゲノム不安定性 / ヒト多能性幹細胞 / 異数染色体 |
|---|
| Outline of Final Research Achievements |
Human pluripotent stem cells, including embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), hold huge potential as reproductive resources for cell-therapies to treat a variety of diseases as well as for drug discovery. In this study, we explored biological function of ABR, a regulator of Rho-family small GTPases, in hESCs. We found that RNAi-mediated depletion of ABR caused several defects in mitotic process, leading to anueploidy transformation with a high frequency. This indicates that ABR has a pivotal role in mitotic fidelity and precise inheritance of genetic information in self-renewing hESCs. Since the emergence of genetically detrimental cells during long-term culture has been regarded to be a big obstacle for the practical utilization of hESCs/iPSCs, these results provide implications for developing hESC/iPSC culture methods that are better suited for human genetic studies and cell-based therapies.
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