Functional analysis of WTAP protein complex on alternative splicing

Research Project

Project/Area Number	26830124
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Genome biology
Research Institution	The University of Tokyo
Principal Investigator	Horiuchi Keiko 東京大学, 先端科学技術研究センター, 助教 (00456203)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	RNAスプライシング / WTAP / 細胞周期 / SUV420H2 / オルタナティブスプライシング / RNAseq / RNA seq
Outline of Final Research Achievements	Wilms’ tumor 1-associating protein (WTAP) is a putative splicing regulator which is required for mouse early embryo development and cell cycle progression. We previously isolated the proteins which interact with WTAP, including VIRILIZER, CBLL1, KIAA0853, RBM15, BCLAF1, THRAP3 and general splicing regulators.To identify the alternative RNA splicing regulated by WTAP protein complex, we performed high-throughput mRNA sequencing using WTAP knockdown cells. Interestingly, WTAP regulates alternative splicing of histone H4 Lys 20 methyltransferases SUV420H1 and SUV420H2 by promoting a production of the truncated isoforms, leading to a change in the global H4K20 methylation level.

Report

(3 results)