Comparative genomics and phenome analysis of closely related Acetic acid bacteria

• Project/Area Number: 26830126
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Yamaguchi University
• Principal Investigator: Matsutani Minenosuke 山口大学, 創成科学研究科, 学術研究員 (70380558)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 同一種多菌株比較ゲノム / 酢酸菌 / 表現型解析 / Acetobacter pasteurianus / ドラフトゲノム解析 / 同一種多菌種間比較ゲノム / Acetobacer pasteurianus / 同一種多株間比較ゲノム

Outline of Final Research Achievements

Acetic acid bacteria (AAB) is industrially used for the production of vinegar. Although many closely related strains have been reported, these strains have different phenotypic characteristics. To understand the genetic background underpinning their phenotypes, the genome sequencing of nine closely related AAB strains was performed, and compared with their phenotypes. As a result, strain isolated from Thailand, SKU1108, was most closely related to strain isolated from Africa and showed highest thermotolerant phenotype, suggesting that their strains adapted to tropical niches. It was suggested that their thermotolerant phenotype is directly affected by their thermotolerant genes.

Research Products

• [Journal Article] Complete Genome Sequencing and Comparative Genomic Analysis of the Thermotolerant Acetic Acid Bacterium, <i>Acetobacter pasteurianus</i> SKU1108, Provide a New Insight into Thermotolerance (2016)
  • Author(s): Matsutani, M., Hirakawa, H., Hiraoka, E., Theeragool, G., Yakushi, T. and Matsushita, K.
  • Journal Title: Microbes and Environments Volume: 31 Issue: 4 Pages: 395-400
  • DOI: 10.1264/jsme2.ME16023
  • NAID: 130005434990
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains. (2016)
  • Author(s): Sainz, F., Jesus Torija, M., Matsutani, M., Kataoka, N., Yakushi, T., Matsushita, K. and Mas, A.
  • Journal Title: Frontiers in Microbiology Volume: 7 Pages: 1358-1358
  • DOI: 10.3389/fmicb.2016.01358
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Adaptive mutation related to cellulose producibility in Komagataeibacter medellinensis (Gluconacetobacter xylinus) NBRC 3288. (2015)
  • Author(s): Matsutani M, Ito K, Azuma Y, Ogino H, Shirai M, Yakushi T, Matsushita K.
  • Journal Title: Appl Microbiol Biotechnol Volume: 99 Issue: 17 Pages: 7229-7240
  • DOI: 10.1007/s00253-015-6598-x
  • Peer Reviewed / Open Access
• [Journal Article] A functionally critical single nucleotide polymorphism in the gene encoding the membrane-bound alcohol dehydrogenase found in ethanol oxidation-deficient Gluconobacter thailandicus (2015)
  • Author(s): P Charoenyingcharoen, M Matsutani, T Yakushi, G Theeragool, P Yukphan, K Matsushita
  • Journal Title: Gene Volume: in press Issue: 2 Pages: 3552-3560
  • DOI: 10.1016/j.gene.2015.04.080
  • Peer Reviewed / Open Access
• [Journal Article] A novel Na+(K+)/H+ antiporter plays an important role in the growth of Acetobacter tropicalis SKU1100 at high temperatures via regulation of cation and pH homeostasis. (2015)
  • Author(s): Soemphol W, Tatsuno M, Okada T, Matsutani M, Kataoka N, Yakushi T, Matsushita K.
  • Journal Title: J Biotechnol. Volume: 211 Pages: 46-55
  • DOI: 10.1016/j.jbiotec.2015.06.397
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Efficient production of 2,5-diketo-D-gluconate via heterologous expression of 2-keto-D-gluconate dehydrogenase in Gluconobacter japonicus. (2015)
  • Author(s): Kataoka N, Matsutani M, Yakushi T, Matsushita K.
  • Journal Title: Appl. Environ. Microbiol. Volume: 81(10) Issue: 10 Pages: 3552-3560
  • DOI: 10.1128/aem.04176-14
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Replacement of a terminal cytochrome c oxidase by ubiquinol oxidase during the evolution of acetic acid bacteria (2014)
  • Author(s): Matsutani M, Fukushima K, Kayama C, Arimitsu M, Hirakawa H, Toyama H, Adachi O, Yakushi T, Matsushita K.
  • Journal Title: Biochim Biophys Acta Volume: 1837 Issue: 10 Pages: 1810-1820
  • DOI: 10.1016/j.bbabio.2014.05.355
  • Peer Reviewed
• [Journal Article] Draft genome sequence of Gluconobacter thailandicus NBRC 3257 (2014)
  • Author(s): Matsutani M, Suzuki H, Yakushi T, Matsushita K.
  • Journal Title: Standards in Genomic Sciences Volume: 9 Issue: 3 Pages: 614-623
  • DOI: 10.4056/sigs.4778605
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of a type II 3-dehydroquinate dehydratase enhances the biotransformation of quinate to 3-dehydroshikimate in Gluconobacter oxydans (2014)
  • Author(s): Nishikura-Imamura S, Matsutani M, Insomphun C, Vangnai AS, Toyama H, Yakushi T, Abe T, Adachi O, Matsushita K.
  • Journal Title: Appl Microbiol Biotechnol Volume: 98 Issue: 7 Pages: 2955-2963
  • DOI: 10.1007/s00253-013-5439-z
  • Peer Reviewed
• [Presentation] 酢酸菌Acetobacter pasteurianus SKU1108の高温適応化株TH-3の発現解析から見える耐熱化機構の解明 (2017)
  • Author(s): 松谷 峰之介、西倉 慎顕、秦野 智行、Saichana Natsaran、Masud-Tippayasak Uraiwan、服部 浩美、貝沼(岡本) 章子、兼崎 友、石川 森男、片岡 尚也、薬師 寿治、松下 一信
  • Organizer: 第11回ゲノム微生物学会・総会
  • Place of Presentation: 神奈川県藤沢市（慶応義塾大学湘南藤沢キャンパス）
  • Year and Date: 2017-03-02
• [Presentation] 酢酸菌Acetobacter pasteurianus 12株のゲノム情報と耐熱性との相関に関する解析 (2016)
  • Author(s): 松谷峰之介、山下隆司、古川藍子、辰野真木、貝沼（岡本）章子、 石川森夫、志波優、吉川博文、平川英樹、薬師寿治、松下一信
  • Organizer: 第10回日本ゲノム微生物学会年会
  • Place of Presentation: 東京工業大学・大岡山キャンパス（東京都目黒区）
  • Year and Date: 2016-03-04
• [Presentation] ゲノムからみた酢酸菌の耐熱性と耐熱化 (2016)
  • Author(s): 松谷峰之介
  • Organizer: 酢酸菌研究会・第８回研究集会
  • Place of Presentation: 和歌山県紀の川市・近畿大学 生物理工学部キャンパス
  • Invited
• [Presentation] 耐熱性酢酸菌Acetobacter pasteurianus SKU1108 (NBRC 101655) 株の 完全長ゲノム配列の決定および比較ゲノム解析 (2015)
  • Author(s): 松谷 峰之介、平川 英樹、薬師 寿治、松下 一信
  • Organizer: 第７回酢酸菌研究会
  • Place of Presentation: 東京大学農学部・中島記念ホール（東京都文京区）
  • Year and Date: 2015-10-10
• [Presentation] 酢酸菌Komagataeibacter medellinensis NBRC 3288株のセルロース非生産株から生産株への復帰機構の解明 (2015)
  • Author(s): 松谷峰之介，伊藤光平，東慶直，荻野英賢，白井睦訓，薬師寿治，松下一信
  • Organizer: 第９回ゲノム微生物学会・総会
  • Place of Presentation: 兵庫県神戸市・神戸大学（六甲第二キャンパス）神大会館
  • Year and Date: 2015-03-06 – 2015-03-08
• [Presentation] 酢酸菌Komagataeibacter medellinensis NBRC 3288のセルロース非生産株から生産株への適応変異 (2014)
  • Author(s): 松谷峰之介，伊藤光平，東慶直，荻野英賢，白井睦訓，薬師寿治，松下一信
  • Organizer: 第6回酢酸菌研究会
  • Place of Presentation: 神奈川県藤沢市・日本大学藤沢キャンパス 生物資源科学部 本館４階大講堂
  • Year and Date: 2014-12-05