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Comparative genomics and phenome analysis of closely related Acetic acid bacteria

Research Project

Project/Area Number 26830126
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Genome biology
Research InstitutionYamaguchi University

Principal Investigator

Matsutani Minenosuke  山口大学, 創成科学研究科, 学術研究員 (70380558)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords同一種多菌株比較ゲノム / 酢酸菌 / 表現型解析 / Acetobacter pasteurianus / ドラフトゲノム解析 / 同一種多菌種間比較ゲノム / Acetobacer pasteurianus / 同一種多株間比較ゲノム
Outline of Final Research Achievements

Acetic acid bacteria (AAB) is industrially used for the production of vinegar. Although many closely related strains have been reported, these strains have different phenotypic characteristics. To understand the genetic background underpinning their phenotypes, the genome sequencing of nine closely related AAB strains was performed, and compared with their phenotypes. As a result, strain isolated from Thailand, SKU1108, was most closely related to strain isolated from Africa and showed highest thermotolerant phenotype, suggesting that their strains adapted to tropical niches. It was suggested that their thermotolerant phenotype is directly affected by their thermotolerant genes.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015 2014

All Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Complete Genome Sequencing and Comparative Genomic Analysis of the Thermotolerant Acetic Acid Bacterium, <i>Acetobacter pasteurianus</i> SKU1108, Provide a New Insight into Thermotolerance2016

    • Author(s)
      Matsutani, M., Hirakawa, H., Hiraoka, E., Theeragool, G., Yakushi, T. and Matsushita, K.
    • Journal Title

      Microbes and Environments

      Volume: 31 Issue: 4 Pages: 395-400

    • DOI

      10.1264/jsme2.ME16023

    • NAID

      130005434990

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains.2016

    • Author(s)
      Sainz, F., Jesus Torija, M., Matsutani, M., Kataoka, N., Yakushi, T., Matsushita, K. and Mas, A.
    • Journal Title

      Frontiers in Microbiology

      Volume: 7 Pages: 1358-1358

    • DOI

      10.3389/fmicb.2016.01358

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Adaptive mutation related to cellulose producibility in Komagataeibacter medellinensis (Gluconacetobacter xylinus) NBRC 3288.2015

    • Author(s)
      Matsutani M, Ito K, Azuma Y, Ogino H, Shirai M, Yakushi T, Matsushita K.
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 99 Issue: 17 Pages: 7229-7240

    • DOI

      10.1007/s00253-015-6598-x

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A functionally critical single nucleotide polymorphism in the gene encoding the membrane-bound alcohol dehydrogenase found in ethanol oxidation-deficient Gluconobacter thailandicus2015

    • Author(s)
      P Charoenyingcharoen, M Matsutani, T Yakushi, G Theeragool, P Yukphan, K Matsushita
    • Journal Title

      Gene

      Volume: in press Issue: 2 Pages: 3552-3560

    • DOI

      10.1016/j.gene.2015.04.080

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel Na+(K+)/H+ antiporter plays an important role in the growth of Acetobacter tropicalis SKU1100 at high temperatures via regulation of cation and pH homeostasis.2015

    • Author(s)
      Soemphol W, Tatsuno M, Okada T, Matsutani M, Kataoka N, Yakushi T, Matsushita K.
    • Journal Title

      J Biotechnol.

      Volume: 211 Pages: 46-55

    • DOI

      10.1016/j.jbiotec.2015.06.397

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Efficient production of 2,5-diketo-D-gluconate via heterologous expression of 2-keto-D-gluconate dehydrogenase in Gluconobacter japonicus.2015

    • Author(s)
      Kataoka N, Matsutani M, Yakushi T, Matsushita K.
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 81(10) Issue: 10 Pages: 3552-3560

    • DOI

      10.1128/aem.04176-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Replacement of a terminal cytochrome c oxidase by ubiquinol oxidase during the evolution of acetic acid bacteria2014

    • Author(s)
      Matsutani M, Fukushima K, Kayama C, Arimitsu M, Hirakawa H, Toyama H, Adachi O, Yakushi T, Matsushita K.
    • Journal Title

      Biochim Biophys Acta

      Volume: 1837 Issue: 10 Pages: 1810-1820

    • DOI

      10.1016/j.bbabio.2014.05.355

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Draft genome sequence of Gluconobacter thailandicus NBRC 32572014

    • Author(s)
      Matsutani M, Suzuki H, Yakushi T, Matsushita K.
    • Journal Title

      Standards in Genomic Sciences

      Volume: 9 Issue: 3 Pages: 614-623

    • DOI

      10.4056/sigs.4778605

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overexpression of a type II 3-dehydroquinate dehydratase enhances the biotransformation of quinate to 3-dehydroshikimate in Gluconobacter oxydans2014

    • Author(s)
      Nishikura-Imamura S, Matsutani M, Insomphun C, Vangnai AS, Toyama H, Yakushi T, Abe T, Adachi O, Matsushita K.
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 98 Issue: 7 Pages: 2955-2963

    • DOI

      10.1007/s00253-013-5439-z

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 酢酸菌Acetobacter pasteurianus SKU1108の高温適応化株TH-3の発現解析から見える耐熱化機構の解明2017

    • Author(s)
      松谷 峰之介、西倉 慎顕、秦野 智行、Saichana Natsaran、Masud-Tippayasak Uraiwan、服部 浩美、貝沼(岡本) 章子、兼崎 友、石川 森男、片岡 尚也、薬師 寿治、松下 一信
    • Organizer
      第11回ゲノム微生物学会・総会
    • Place of Presentation
      神奈川県藤沢市(慶応義塾大学湘南藤沢キャンパス)
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] 酢酸菌Acetobacter pasteurianus 12株のゲノム情報と耐熱性との相関に関する解析2016

    • Author(s)
      松谷峰之介、山下隆司、古川藍子、辰野真木、貝沼(岡本)章子、 石川森夫、志波優、吉川博文、平川英樹、薬師寿治、松下一信
    • Organizer
      第10回日本ゲノム微生物学会年会
    • Place of Presentation
      東京工業大学・大岡山キャンパス(東京都目黒区)
    • Year and Date
      2016-03-04
    • Related Report
      2015 Research-status Report
  • [Presentation] ゲノムからみた酢酸菌の耐熱性と耐熱化2016

    • Author(s)
      松谷峰之介
    • Organizer
      酢酸菌研究会・第8回研究集会
    • Place of Presentation
      和歌山県紀の川市・近畿大学 生物理工学部キャンパス
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 耐熱性酢酸菌Acetobacter pasteurianus SKU1108 (NBRC 101655) 株の 完全長ゲノム配列の決定および比較ゲノム解析2015

    • Author(s)
      松谷 峰之介、平川 英樹、薬師 寿治、松下 一信
    • Organizer
      第7回酢酸菌研究会
    • Place of Presentation
      東京大学農学部・中島記念ホール(東京都文京区)
    • Year and Date
      2015-10-10
    • Related Report
      2015 Research-status Report
  • [Presentation] 酢酸菌Komagataeibacter medellinensis NBRC 3288株のセルロース非生産株から生産株への復帰機構の解明2015

    • Author(s)
      松谷峰之介,伊藤光平,東慶直,荻野英賢,白井睦訓,薬師寿治,松下一信
    • Organizer
      第9回ゲノム微生物学会・総会
    • Place of Presentation
      兵庫県神戸市・神戸大学(六甲第二キャンパス)神大会館
    • Year and Date
      2015-03-06 – 2015-03-08
    • Related Report
      2014 Research-status Report
  • [Presentation] 酢酸菌Komagataeibacter medellinensis NBRC 3288のセルロース非生産株から生産株への適応変異2014

    • Author(s)
      松谷峰之介,伊藤光平,東慶直,荻野英賢,白井睦訓,薬師寿治,松下一信
    • Organizer
      第6回酢酸菌研究会
    • Place of Presentation
      神奈川県藤沢市・日本大学藤沢キャンパス 生物資源科学部 本館4階大講堂
    • Year and Date
      2014-12-05
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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