| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We investigated whether PI polyamides designed targeting of the TGF-β1 have the effect regulation of EMT/MET, and increase the human iPS cell induction efficiency. We designed and synthesized three different types of PI polyamides designed targeting of the TGF-β1. We were administered PI polyamide to human fibroblasts transfected with exogenous reprogramming factor by Sendai virus vector and grown in human iPS induction cultures were seeded on feeder cells, was observed a significant increase in alkaline phosphatase-positive colony number of PI polyamide treated group compared with the non-treated group, we also found that the expression of undifferentiated markers was observed in these colonies. It was found to regulate of EMT/MET, and increase the human iPS cell induction efficiency in human fibroblasts by PI polyamides targeting human TGF-beta gene promoter region.
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