Functional network of nascent protein and molecular chaperones
Project/Area Number |
26830141
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
System genome science
|
Research Institution | University of Hyogo |
Principal Investigator |
Machida Kodai 兵庫県立大学, 工学研究科, 助教 (20553093)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 合成生物学 / 試験内タンパク質合成 / 分子シャペロン / 新生鎖 / フォールディング / 真核生物 / タンパク質合成 / タンパク質フォールディング / シャペロン |
Outline of Final Research Achievements |
In this study, we established an in vitro protein synthesis/folding coupled system reconstituted with purified human translational factors and molecular chaperones. Using this system, we successfully identify the molecular chaperones that are essential for co- or post-translational folding of human nascent proteins. Thus, our in vitro approach can be a useful tool to analyze the molecular mechanisms of protein conformational disorders such as Alzheimer’s disease, Parkinson's disease and Huntington's disease.
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Report
(4 results)
Research Products
(22 results)