Epidemiological risk prediction and validation of fungal resistance mechanism of threatened amphibian species based on immunity related genes

• Project/Area Number: 26830144
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservation of biological resources
• Research Institution: Hiroshima University
• Principal Investigator: Igawa Takeshi 広島大学, 国際協力研究科, 助教 (00507197)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 両生類 / 絶滅危惧種 / 西南諸島 / 主要組織適合遺伝子複合体 / MHC / トランスクリプトーム / 発現解析 / 分子進化 / RNA-seq / 免疫 / 病原体 / 集団遺伝 / 遺伝的多様性

Outline of Final Research Achievements

For validation of diversity and molecular evolution of immunity related genes which contribute to resist to pathogenic microorganisms such as fungal diseases by batrachochytrium dendrobatidis, we identified major histocompatibility complex (MHC) and natural immune system related genes in endangered amphibian species (Ishikawa’s frogs, Otton frog, Holsti’s frog, Ryukyu tip-nosed frogs and crocodile newt) distributing in South-Western islands in Japan. As the results of this study, we identified multiple MHC I and II genes and natural immunity related genes in all six species and also elucidated diversities and different expression patterns among different species.

Research Products

• [Journal Article] Fine-scale demographic processes resulting from multiple overseas colonization events of the Japanese stream tree frog, Buergeria japonica (2017)
  • Author(s): Shohei Komaki, Si-Min Lin, Masafumi Nozawa, Shohei Oumi, Masayuki Sumida, Takeshi Igawa
  • Journal Title: Journal of Biogeography Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Development and Characterization of 23 Microsatellite Markers for the Montane Brown Frog (<i>Rana ornativentris</i>) (2017)
  • Author(s): Hisanori Okamiya, Takeshi Igawa, Masafumi Nozawa, Masayuki Sumida, and Tamotsu Kusano
  • Journal Title: Current Herpetology Volume: 36 Issue: 1 Pages: 63-68
  • DOI: 10.5358/hsj.36.63
  • NAID: 130005398694
  • ISSN: 1345-5834, 1881-1019
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Salinity and thermal tolerance of Japanese stream tree frog (Buergeria japonica) tadpoles from island populations (2016)
  • Author(s): Shohei Komaki, Takeshi Igawa, Si-Min Lin, Masayuki Sumida
  • Journal Title: Herpetological Journal Volume: 26 Pages: 207-211
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Inter- and intra-specific genetic divergence of Asian tiger frogs (genus <i>Hoplobatrachus</i>), with special reference to the population structure of <i>H. tigerinus</i> in Bangladesh (2016)
  • Author(s): Sultana Nasrin, Takeshi Igawa, Mohammed Mafizul Islam, Mahmudul Hasan, Mohammad Shafiqul Alam, Shohei Komaki, Kensuke Kawamura, Md. Mukhlesur Rahman Khan, Masayuki Sumida
  • Journal Title: Genes & Genetic Systems Volume: 91 Issue: 4 Pages: 217-227
  • DOI: 10.1266/ggs.16-00006
  • NAID: 130005450119
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The first see-through frog created by breeding: description, inheritance patterns, and dermal chromatophore structure (2016)
  • Author(s): Masayuki Sumida, Mohammed Islam, Takeshi Igawa, Atsushi Kurabayashi, Yukari Furukawa, Naomi Sano, Tamotsu Fujii, Norio Yoshizaki
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 24431-24431
  • DOI: 10.1038/srep24431
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Population structure and evolution after speciation of the Hokkaido salamander (Hynobius retardatus) (2016)
  • Author(s): Masatoshi Matsunami, Takeshi Igawa, Hirofumi Michimae, Toru Miura, Kinya Nishimura
  • Journal Title: PLoS One Volume: 11 Issue: 6 Pages: e0156815-e0156815
  • DOI: 10.1371/journal.pone.0156815
  • NAID: 120005859706
  • Peer Reviewed / Open Access
• [Journal Article] Living in a Japanese onsen: field observations and physiological measurements of hot spring amphibian tadpoles, Buergeria japonica (2016)
  • Author(s): Shohei Komaki, Lau Quintin, Takeshi Igawa
  • Journal Title: Amphibia-Reptilia Volume: 37 Issue: 3 Pages: 311-314
  • DOI: 10.1163/15685381-00003052
  • Peer Reviewed / Open Access
• [Journal Article] Characterisation of major histocompatibility complex class I genes in Japanese Ranidae frogs (2016)
  • Author(s): Lau, Quintin, Igawa, Takeshi, Komaki, Shohei, Satta, Yoko
  • Journal Title: Immunogenetics Volume: 68 Issue: 10 Pages: 797-806
  • DOI: 10.1007/s00251-016-0934-x
  • NAID: 120006629851
  • Peer Reviewed / Open Access
• [Journal Article] Salinity and thermal tolerance of Japanese stream tree frog (Buergeria japonica) tadpoles from island populations. (2016)
  • Author(s): Shohei Komaki, Takeshi Igawa, Si-Min Lin, Masayuki Sumida
  • Journal Title: Herpetological Journal Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Inter- and intra-specific genetic divergence of Asian tiger frogs (genus Hoplobatrachus), with special reference to the population structure of H. tigerinus in Bangladesh. (2016)
  • Author(s): Sultana Nasrin, Takeshi Igawa, Mohammed Mafizul Islam, Mahmudul Hasan, Mohammad Shafiqul Alam, Shohei Komaki, Kensuke Kawamura, Md. Mukhlesur Rahman Khan, and Masayuki Sumida
  • Journal Title: Genes & Genetic Systems Volume: 印刷中
  • NAID: 130005450119
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Extraordinary diversity in the origins of sex chromosomes in anurans inferred from comparative gene mapping. (2015)
  • Author(s): Yoshinobu Uno, Chizuko Nishida, Chiyo Takagi, Takeshi Igawa, Naoto Ueno, Masayuki Sumida, and Yoichi Matsuda
  • Journal Title: Cytogenetic and Genome Research Volume: 145 Issue: 3-4 Pages: 218-229
  • DOI: 10.1159/000431211
  • Peer Reviewed
• [Journal Article] Radiocesium accumulation in the anuran frog, Rana tagoi tagoi, in forest ecosystems after the Fukushima Nuclear Power Plant accident (2015)
  • Author(s): T. Takahara, S. Endo, M. Takada a, Y. Oba, W. I. Nursal, T. Igawa, H. Doi, T. Yamada, T. Okuda
  • Journal Title: Environm. Pol. Volume: 199 Pages: 89-94
  • DOI: 10.1016/j.envpol.2015.01.018
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic population structure and demographic history of an endangered frog, Babina holsti (2015)
  • Author(s): Kakehashi R., T. Igawa, and M. Sumida
  • Journal Title: Conservation Genetics Volume: 16 Issue: 4 Pages: 987-1000
  • DOI: 10.1007/s10592-015-0718-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development and Characterization of 12 Microsatellite Markers for the Hokkaido Salamander (<i>Hynobius retardatus</i>) (2015)
  • Author(s): Masatoshi Matsunami, Takeshi Igawa, Masafumi Nozawa, Hirofumi Michimae, Toru Miura, and Kinya Nishimura
  • Journal Title: Current Herpetology Volume: 34 Issue: 2 Pages: 177-181
  • DOI: 10.5358/hsj.34.177
  • NAID: 130005095175
  • ISSN: 1345-5834, 1881-1019
  • Peer Reviewed
• [Journal Article] Robust molecular phylogeny and palaeodistribution modelling resolve a complex evolutionary history: glacial cycling drove recurrent mtDNA introgression among Pelophylax frogs in East Asia. (2015)
  • Author(s): Shohei Komaki, Takeshi Igawa, Si-Min Lin, Koji Tojo, Mi-Sook Min, and Masayuki Sumida
  • Journal Title: Journal of Biogeography Volume: 42 Issue: 11 Pages: 2159-2171
  • DOI: 10.1111/jbi.12584
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Inbreeding ratio and genetic relationships among strains of the Western clawed frog, Xenopus tropicalis. (2015)
  • Author(s): Takeshi Igawa, Ai Watanabe, Atsushi Suzuki, Akihiko Kashiwagi, Keiko Kashiwagi, Anna Noble, Matt Guille, David E. Simpson, Marko E. Horb, Tamotsu Fujii, and Masayuki Sumida
  • Journal Title: PLoS One Volume: 10 Issue: 7 Pages: e0133963-e0133963
  • DOI: 10.1371/journal.pone.0133963
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Development and Validation of PCR-RFLP Assay to Identify Three Japanese Brown Frogs of the True Frog Genus <i>Rana</i> (2015)
  • Author(s): Igawa T., S. Komaki, T. Takahara, and M. Sumida
  • Journal Title: Current Herpetology Volume: 34 Issue: 1 Pages: 89-94
  • DOI: 10.5358/hsj.34.89
  • NAID: 130004888109
  • ISSN: 1345-5834, 1881-1019
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Extraordinary diversity in the origins of sex chromosomes in anurans inferred from comparative gene mapping (2015)
  • Author(s): Yoshinobu Uno, Chizuko Nishida, Chiyo Takagi, Takeshi Igawa, Naoto Ueno, Masayuki Sumida, and Yoichi Matsuda
  • Journal Title: Cytogenetic and Genome Research Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Genetic divergences of South and Southeast Asian frogs : a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya (2014)
  • Author(s): Mahmudul Hasan, Mohammed Mafizul Islam, Mukhlesur Rahman Khan, and Takeshi Igawa, Mohammad Shafiqul Alam, HonTjong Djong, Nia Kurniawan, Hareesh Joshy, Yong Hoi Sen, Daicus M. Belabut, Atsushi Kurabayashi, Mitsuru Kuramoto, Masayuki Sumida
  • Journal Title: Turkish Journal of Zoology Volume: 38 Pages: 1-22
  • DOI: 10.3906/zoo-1308-36
  • Peer Reviewed / Open Access
• [Journal Article] Development and characterization of 14 microsatellite markers for <i>Buergeria japonica</i> (Amphibia, Anura, Rhacophoridae) (2014)
  • Author(s): Shohei Komaki, Takeshi Igawa, Masafumi Nozawa, Si-Min Lin, Shohei Oumi, and Masayuki Sumida
  • Journal Title: Genes & Genetic Systems Volume: 89 Issue: 1 Pages: 35-39
  • DOI: 10.1266/ggs.89.35
  • NAID: 130004137821
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access
• [Journal Article] Development and characterization of 27 new microsatellite markers for the Indian bullfrog <i>Hoplobatrachus tigerinus</i> and its congeneric species (2014)
  • Author(s): Nasrin S., T. Igawa, M. Nozawa, M. M. Islam, M. Hasan, M. S. Alam, M. M. R. Khan, and M. Sumida
  • Journal Title: Genes & Genetic Systems Volume: 89 Issue: 3 Pages: 137-141
  • DOI: 10.1266/ggs.89.137
  • NAID: 130004703732
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic Divergence and Geographic Distribution of Frogs in Genus Fejervarya from Indonesia Inferred from Mitochondrial 16S rRNA Gene Analysis (2014)
  • Author(s): Nia Kuruniawan, Tjong Hon Djong, Tesri Maideliza, Amir Hamidy, Mahumudul Hasan, Takeshi Igawa, and Masayuki Sumida
  • Journal Title: Treubia Volume: 41 Pages: 1-16
  • Peer Reviewed / Open Access
• [Journal Article] Microsatellite Marker development by Multiplex Ion Torrent PGM Sequencing: A case study of the endangered Odorrana narina complex of frogs (2014)
  • Author(s): Igawa T., M. Nozawa, M. Nagaoka, S. Komaki, S Oumi, T Fujii, and M Sumida
  • Journal Title: Journal of Heredity Volume: 106 Issue: 1 Pages: 131-137
  • DOI: 10.1093/jhered/esu071
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Identifying immune genes from Japanese frogs (Genus Rana) (2016)
  • Author(s): Quintin Lau, Takeshi Igawa, Yoko Satta
  • Organizer: Joint meeting of the 22nd International Congress of Zoology (ICZ) & the 87th meeting of the ZSJ
  • Place of Presentation: 恩納村 沖縄県
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Genetic relationships and inbreeding ratio among strains of the western clawed frog, Xenopus tropicalis (2016)
  • Author(s): Takeshi Igawa, Ai Watanabe, Atsushi Suzuki, Akihiko Kashiwagi, Keiko Kashiwagi, Anna Noble, Matt Guille, David E. Simpson, Marko E. Horb, Tamotsu Fujii
  • Organizer: Joint meeting of the 22nd International Congress of Zoology (ICZ) & the 87th meeting of the ZSJ
  • Place of Presentation: 恩納村 沖縄県
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Comparative transcriptome analyses of eight endangered amphibians in Ryukyu Archipelago (2016)
  • Author(s): Takeshi Igawa, Masatoshi Matsunami, Quintin Lau, Masayuki Sumida
  • Organizer: The 8th World Congress of Herpetology
  • Place of Presentation: Tonglu, China
  • Year and Date: 2016-08-15
  • Int'l Joint Research
• [Presentation] Comparative transcriptome analyses of eight endangered amphibians in Ryukyu Archipelago (2016)
  • Author(s): Takeshi Igawa, Masatoshi Matsunami, Quintin Lau, Masayuki Sumida
  • Organizer: Society for Molecular Biology and Evolution (SMBE)
  • Place of Presentation: Gold Coast, Australia
  • Year and Date: 2016-07-03
  • Int'l Joint Research
• [Presentation] Selection acting on MHC class I genes in Japanese Ranidae frogs (2016)
  • Author(s): Quintin Lau, Takeshi Igawa, Shohei Komaki, Yoko Satta
  • Organizer: Society for Molecular Biology and Evolution (SMBE)
  • Place of Presentation: Gold Coast, Australia
  • Year and Date: 2016-07-03
  • Int'l Joint Research
• [Presentation] リュウキュウカジカガエルの集団動態 (2015)
  • Author(s): 小巻翔平・井川武・住田正幸・野澤昌文・林思民
  • Organizer: 日本爬虫両生類学会第54回大会
  • Place of Presentation: 東邦大学（千葉県・船橋市）
  • Year and Date: 2015-12-05
• [Presentation] Characterisation of major histocompatibility complex class II （MHC II）in Japanese frogs. (2015)
  • Author(s): Quintin Lau, Takeshi Igawa, Yoko Satta
  • Organizer: 日本遺伝学会第87回大会
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2015-09-24
• [Presentation] 次世代モデルリソース・ネッタイツメガエル7系統における近交度及び遺伝的関係 (2015)
  • Author(s): 渡辺愛・井川武・柏木昭彦・鈴木厚・倉林敦・藤井保・住田正幸
  • Organizer: 第一回次世代両生類研究会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県・岡崎市）
  • Year and Date: 2015-08-24
• [Presentation] 生命科学研究における近交系ネッタイツメガエルの有用性 (2015)
  • Author(s): 柏木昭彦・柏木啓子・花田秀樹・鈴木賢一・鈴木厚・竹林公子・倉林敦・中島圭介・田澤一朗・井川武・古野伸明・山本卓・住田正幸
  • Organizer: 第一回次世代両生類研究会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県・岡崎市）
  • Year and Date: 2015-08-24
• [Presentation] ツメガエル類に関するさまざまな実験例 (2015)
  • Author(s): 柏木昭彦・柏木啓子・花田秀樹・鈴木賢一・鈴木厚・竹林公子・倉林敦・中島圭介・田澤一朗・井川武・古野伸明・山本卓・住田正幸
  • Organizer: 第一回次世代両生類研究会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県・岡崎市）
  • Year and Date: 2015-08-24
• [Presentation] Fine scale genetic divergence among Hoplobatrachus tigernus from Bangladesh and neighboring countries elucidated by mitochondrial genes and microsatellite markers (2014)
  • Author(s): Nasrin Sultana, Takeshi Igawa, Masafumi Nozawa, Mohammed Mafizul Islam, Mahmudul Hsan, Mohammad Shafiqul Alam, Md. Mukhlesur Rahman Khan, Masayuki Sumida
  • Organizer: 日本爬虫両生類学会第53回大会
  • Place of Presentation: 神戸市
  • Year and Date: 2014-11-08 – 2014-11-09
• [Presentation] 次世代モデルリソース・ネッタイツメガエル7系統における近交度及び遺伝的関係 (2014)
  • Author(s): 渡辺愛・井川武・柏木昭彦・鈴木厚・倉林敦・藤井保・住田正幸
  • Organizer: 日本爬虫両生類学会第53回大会
  • Place of Presentation: 神戸市
  • Year and Date: 2014-11-08 – 2014-11-09
• [Presentation] Conservation genetics of endangered amphibians in Ryukyu Archipelago (2014)
  • Author(s): Takeshi Igawa
  • Organizer: "MHC evolution and Human evolution" symposium
  • Place of Presentation: 三浦郡葉山町
  • Year and Date: 2014-07-26 – 2014-07-27
  • Invited