Molecular mechanism of genome maintenance by histone H1 ubiquitination

• Project/Area Number: 26840003
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: University of Tsukuba
• Principal Investigator: Kato Kohsuke 筑波大学, 医学医療系, 助教 (90466673)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ゲノム安定性 / ヒストンH1 / ユビキチン / クロマチン / G1-S遺伝子群 / 転写 / G1/S遺伝子群 / 遺伝子 / 細胞周期

Outline of Final Research Achievements

Aim of this research is to reveal the molecular mechanism of genome maintenance through the VprBP-mediated histone H1 ubiquitination. Depletion of VprBP caused the accumulation of DNA damage and cell cycle arrest at late S-G2 phase in cells. We found that VprBP is involved in transcription elongation of activating E2F and homologous recombination-repair genes in histone H1-dependent manner. These results raise a possibility that VprBP-mediated histone H1 ubiquitination is involved in genome maintenance through the transcriptional regulation of genes activated in cell cycle G1-S phases.

Research Products

• [Journal Article] Glucocorticoids facilitate the transcription from the human cytomegalovirus major immediate early promoter in glucocorticoid receptor- and nuclear factor-I-like protein-dependent manner. (2015)
  • Author(s): Inoue-Toyoda M, Kato K, Nagata K, Yoshikawa H.
  • Journal Title: Biochem Biophys Res Commun. Volume: 476 Issue: 1 Pages: 9-14
  • DOI: 10.1016/j.bbrc.2015.01.091
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ヒストンH1ユビキチン化によるG1-S遺伝子群の転写制御 (2015)
  • Author(s): 加藤広介、永田恭介
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（神戸市）
  • Year and Date: 2015-12-01