Functional screening of the genes involved in Xist function and pluripotency
| Project/Area Number |
26840006
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | エピジェネティクス / X染色体不活性化 / ノンコーディングRNA / 非コードRNA / X染色体の不活性化 / 長鎖非コードRNA / 多能性細胞 / Xist |
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| Outline of Final Research Achievements |
In the term, I introduced and mated XTTGFP/XTTFP female mice (XTTGFP: Tsix inducible and X-linked GFP allele) with XTX/Y male mice (XTX: Xist inducible allele) to establish XTTGFP/XTX Embryonic stem cells (ESCs). Subsequently, I knocked-in the mCherry gene using CRISPR/Cas9 system into the XTX allele and introduced the Cas9 gene into the ESC to obtain XTTGFP/XTXmcherry Cas9 ESC. The ESC was converted to EpiSC. CRISPR library was amplified, and then the screening system has been established.
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Report
(4 results)
Research Products
(12 results)
