| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Although It is known that heterochromatin (HC) structure that suppresses the gene expression is clustered at nuclear periphery or around the nucleolus, its mechanism and biological significance are not well understood. In this study, I aimed to clarify the mechanism of HC cluster formation using fission yeast as a model organism. A system capable of artificially inducing HC dependent on small RNAs was constructed in the arm of the chromosome. The behavior of this artificially induced HC in the nucleus was analyzed in detail by the 3C method (Chromosome Conformation Capture). As a result, we reveled this artificially induced HC region spatially interacts with the native HC region such as centromere and telomere at the chromatin level. This result clearly showed that the RNAi-dependent HC structure has the ability to form clusters in the nucleus.
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