| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
To investigate elongator tRNA (eMet) localization, we tried things written below. We revealed sequence of DNA/RNA hybrid which fluoresces by RNaseH digestion. Furthermore, molecular beacon (MB) optimized target site on eMet could detec mature eMet effectively.
Formation of tRNA granules was inhibited by knockdown of proteins forming nuclear stress bodies. Nuclear stress bodies colocalized with tRNA granules. In addition, mammalian target of rapamycin is important to nuclear accumulation of eMet.
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