Elucidation of tRNA transport mechanism under heat stress
| Project/Area Number |
26840010
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 伸長tRNA / tRNA顆粒 / nuclear stress bodies / リアルタイムイメージング / 伸長tRNAMet |
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| Outline of Final Research Achievements |
To investigate elongator tRNA (eMet) localization, we tried things written below. We revealed sequence of DNA/RNA hybrid which fluoresces by RNaseH digestion. Furthermore, molecular beacon (MB) optimized target site on eMet could detec mature eMet effectively.
Formation of tRNA granules was inhibited by knockdown of proteins forming nuclear stress bodies. Nuclear stress bodies colocalized with tRNA granules. In addition, mammalian target of rapamycin is important to nuclear accumulation of eMet.
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Report
(3 results)
Research Products
(9 results)
