NMR analyses of the structural equilibrium in tyrosine kinases
| Project/Area Number |
26840014
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Keio University (2015)
Hokkaido University (2014) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | キナーゼ / NMR / チロシンキナーゼ |
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| Outline of Final Research Achievements |
Tyrosine kinases are major targets for anticancer drug. To understand the dynamic regulation of tyrosine kinases, NMR analyses of the structural equilibrium were performed. In this study, E coli. expression system and purification protocol for c-Src, Fyn, and c-Abl were successfully established. NMR spectral changes upon phosphorylation of c-Src and upon binding to drugs were observed. NMR approach would be useful for understanding the dynamic processes in the regulation of tyrosine kinases.
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Report
(3 results)
Research Products
(13 results)
