Grant-in-Aid for Young Scientists (B)
We tried to solve the crystal structure of MsMab-1 and 2. We identified a critical amino acid residue for the multi-specificity from the crystal structure of MsMab-1. Hence MsMab-1 was mutated the amino acid residue of antigen recognition site based on the structure. Mutated MsMab-1 was changed the specificity against mutated IDH1/2. MsMab-2 was not able to try a crystallization experiment for its low level of productivity.We generated a novel multi-specific anti-IDH1/2 monoclonal antibody MsMab-3, which could bind mutated IDH1/2 peptides but not mutated IDH1/2 proteins.
Attribution of KAKENHI