| Project/Area Number |
26840017
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Nakatogawa Machiko 東京工業大学, 生命理工学院, JSPS特別研究員 (90402461)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | オートファジー / ユビキチン様タンパク質 / E2酵素 / 蛍光顕微鏡 / 結晶構造解析 / E3酵素 |
|---|
| Outline of Final Research Achievements |
Autophagy is a lysosome/vacuole-mediated degradation system in eukaryotic cells, involved in various diseases such as neurodegenerative diseases, hepatic diseases, cancer, and diabetes. Formation of double-membrane vesicles “autophagosomes” that enwrap degradation targets is essential for autophagy. In this study, we revealed the localization of the autophagy-related protein Atg3 to autophagosome intermediates under autophagy-inducing conditions, and that the impairment of its localization results in inefficient autophagosome formation. Based on our results, we proposed the mechanism by which Atg3 participate in autophagosome formation.
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