Structural basis of Archaeal ESCRT complex

• Project/Area Number: 26840018
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: University of Toyama
• Principal Investigator: OBITA TAKAYUKI 富山大学, 大学院医学薬学研究部(薬学), 准教授 (30578696)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細胞質分裂 / ESCRT / 構造生物学

Outline of Final Research Achievements

We have determined the structure of two archaeal ESCRT-II like proteins, one is from S. acidocaldarius and the other is from S. solfataricus. Both proteins have winged-helix domain fold with additional two helices, but the structures are quite different from human ESCRT-II proteins. We have also showed that the archaeal ESCRT-II like proteins bound to DNA. These results show that ESCRT proteins may have unique roles in archaea.

Research Products

• [Journal Article] Characterization of the interaction between Trypanosoma brucei Pex5p and its receptor Pex14p. (2016)
  • Author(s): Watanabe Y, Kawaguchi K, Okuyama N, Sugawara Y, Obita T, Mizuguchi M, Morita M, Imanaka T.
  • Journal Title: FEBS letters Volume: 590 Issue: 2 Pages: 242-250
  • DOI: 10.1002/1873-3468.12044
  • Peer Reviewed
• [Journal Article] Structural fine-tuning of MIT interacting motif 2 (MIM2) and allosteric regulation of ESCRT-III by Vps4 in yeast. (2016)
  • Author(s): Kojima R, Obita T, Onoue K, Mizuguchi M.
  • Journal Title: Journal of Molecular Biology Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] In silico and pharmacological screenings identify novel serine racemase inhibitors. (2014)
  • Author(s): Hisashi Mori, Ryogo Wada, Jie Li, Tetsuya Ishimoto, Mineyuki Mizuguchi, Takayuki Obita, Hiroaki Gouda, Shuichi Hirono, Naoki Toyooka.
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 24 Issue: 16 Pages: 3732-3735
  • DOI: 10.1016/j.bmcl.2014.07.003
  • Peer Reviewed
• [Journal Article] Transient α-helices in the disordered RPEL motifs of the serum response factor coactivator MKL1. (2014)
  • Author(s): Mizuguchi M, Fuju T, Obita T, Ishikawa M, Tsuda M, Tabuchi A.
  • Journal Title: Sci Rep. Volume: 4 Issue: 1 Pages: 5224-5224
  • DOI: 10.1038/srep05224
  • Peer Reviewed / Open Access
• [Journal Article] Mutations in the gene PQBP1 prevent its interaction with the spliceosomal protein U5-15kD (2014)
  • Author(s): Mizuguchi, M., Obita, T., Serita, T., Kojima, R., Nabeshima, Y., and Okazawa, H.
  • Journal Title: Nat Commun. Volume: 5 Issue: 1 Pages: 3822-3822
  • DOI: 10.1038/ncomms4822
  • Peer Reviewed
• [Presentation] Vps4によるESCRT-IIIタンパク質の認識に関する研究 (2015)
  • Author(s): 帯田孝之
  • Organizer: 第二回生命分子科学研究会
  • Place of Presentation: 北海道・小樽朝里クラッセホテル
  • Year and Date: 2015-03-14 – 2015-03-16