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Structural and functional analysis of gap junction channels using electron microscopy

Research Project

Project/Area Number 26840019
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionNagoya University

Principal Investigator

Oshima Atsunori  名古屋大学, 名古屋大学細胞生理学研究センター, 准教授 (80456847)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsギャップ結合チャネル / イネキシン / 電子顕微鏡 / 三次元再構成 / 二次元結晶 / 極低温電子顕微鏡 / 二次元結晶化
Outline of Final Research Achievements

In this project, the structure of C. elegans innexin-6 (INX-6) was investigated using electron crystallography. Two dimensional crystals of N-terminal deleted INX-6 where 2 to 19 were eliminated were successfully obtained. The structure of INX-6delN reveals the six-teen subunits, a hexadecamer. The plug density was observed on the channel pore pathway, suggesting a structure in a closed form. These results have been published as a research article

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 5 results)

  • [Journal Article] Hexadecameric structure of an invertebrate gap junction channel2016

    • Author(s)
      Oshima, A., Matsuzawa, T., Murata, K., Tani, K. and Fujiyoshi, Y.
    • Journal Title

      Journal of Molecular Biology

      Volume: 428 Issue: 6 Pages: 1227-1236

    • DOI

      10.1016/j.jmb.2016.02.011

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ギャップ結合チャネルの構造と多様性2016

    • Author(s)
      大嶋篤典
    • Journal Title

      膜

      Volume: 41 Pages: 50-56

    • NAID

      130005249079

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GraDeR: membrane protein complex preparation for single particle cryo-EM.2015

    • Author(s)
      Hauer F., Gerle C., Fischer N., Oshima A., Shinzawa-Itoh K., Shimada S., Yokoyama K., Fujiyoshi Y. & Stark H.
    • Journal Title

      Structure

      Volume: 23 Issue: 9 Pages: 1769-1775

    • DOI

      10.1016/j.str.2015.06.029

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] THREE-DIMENSIONAL STRUCTURE OF INNEXIN GAP JUNCTION CHANNELS STUDIED BY ELECTRON CRYSTALLOGRAPHY2016

    • Author(s)
      Atsunori Oshima, Tomohiro Matsuzawa, Kazuyoshi Murata, Kazutoshi Tani and Yoshinori Fujiyoshi
    • Organizer
      60th Biophysical Society Annual Meeting
    • Place of Presentation
      Los Angeles Convention Center, Los Angeles
    • Year and Date
      2016-02-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Three dimensional structure of C. elegans innexin gap junction channels2015

    • Author(s)
      Atsunori Oshima, Tomohiro Matsuzawa, Kazuyoshi Murata, Kazutoshi Tani and Yoshinori Fujiyoshi
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Initial 3D reconstruction of innexin gap junction channels by electron crystallography2015

    • Author(s)
      Atsunori Oshima,, Tomohiro Matsuzawa, Kazuyoshi Murata, Kazutoshi Tani and Yoshinori Fujiyoshi
    • Organizer
      日本顕微鏡学会 第71回学術講演会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2015-05-13 – 2015-05-15
    • Related Report
      2014 Research-status Report
  • [Presentation] Initial 3D reconstruction of innexin gap junction channels by electron crystallography2015

    • Author(s)
      Atsunori Oshima,, Tomohiro Matsuzawa, Kazuyoshi Murata, Kazutoshi Tani and Yoshinori Fujiyoshi
    • Organizer
      日本顕微鏡学会 第71回学術講演会
    • Place of Presentation
      国立京都国際会館、京都
    • Year and Date
      2015-05-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Structure and diversity of gap junction channels studied by electron microscopy2015

    • Author(s)
      Atsunori Oshima
    • Organizer
      第120回日本解剖学会総会・全国学術集会・第92回日本生理学会大会合同大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Electron Microscopic Studies of Invertebrate Gap Junction Channels2015

    • Author(s)
      Atsunori Oshima, Tomohiro Matsuzawa, Kazuyoshi Murata, Kouki Nishikawa, Kazutoshi Tani and Yoshinori Fujiyoshi,
    • Organizer
      IGER International Symposium on Frontiers in Biological Research with Advanced Electron Microscope Technologies
    • Place of Presentation
      Nagoya University
    • Year and Date
      2015-01-15 – 2015-01-16
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 電子顕微鏡で見るギャップ結合チャネルの構造と多様性2014

    • Author(s)
      大嶋篤典
    • Organizer
      「電子顕微鏡機能イメージングの医学・生物学への応用」~次世代の生物電顕を考える~
    • Place of Presentation
      生理学研究所
    • Year and Date
      2014-11-12 – 2014-11-13
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Electron microscopy of C. elegans innexin-6 gap junction channels indicates the characteristic subunit organization2014

    • Author(s)
      Atsunori Oshima, Tomohiro Matsuzawa, Kazuyoshi Murata, Kouki Nishikawa, and Yoshinori Fujiyoshi
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Oligomeric form and function of C. elegans innexin-6 gap junction channels2014

    • Author(s)
      Atsunori Oshima, Tomohiro Matsuzawa, Kazuyoshi Murata, Kouki Nishikawa, and Yoshinori Fujiyoshi
    • Organizer
      the 23rd International Union of Crystallography (IUCr) Congress and General Assembly
    • Place of Presentation
      Montréal, Québec, Canada
    • Year and Date
      2014-08-05 – 2014-08-12
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 電子顕微鏡によるイネキシンギャップ結合チャネルの構造研究2014

    • Author(s)
      大嶋篤典、松澤朋寛、村田和義、西川幸希、藤吉好則
    • Organizer
      日本顕微鏡学会第70回記念学術講演会
    • Place of Presentation
      幕張メッセ 国際会議場
    • Year and Date
      2014-05-11 – 2014-05-13
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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