Structural and functional analysis of gap junction channels using electron microscopy
| Project/Area Number |
26840019
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
Oshima Atsunori 名古屋大学, 名古屋大学細胞生理学研究センター, 准教授 (80456847)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | ギャップ結合チャネル / イネキシン / 電子顕微鏡 / 三次元再構成 / 二次元結晶 / 極低温電子顕微鏡 / 二次元結晶化 |
|---|
| Outline of Final Research Achievements |
In this project, the structure of C. elegans innexin-6 (INX-6) was investigated using electron crystallography. Two dimensional crystals of N-terminal deleted INX-6 where 2 to 19 were eliminated were successfully obtained. The structure of INX-6delN reveals the six-teen subunits, a hexadecamer. The plug density was observed on the channel pore pathway, suggesting a structure in a closed form. These results have been published as a research article
|
Report
(3 results)
Research Products
(13 results)
-
-
-
-
-
-
-
-
-
[Presentation] Electron Microscopic Studies of Invertebrate Gap Junction Channels2015
Author(s)
Atsunori Oshima, Tomohiro Matsuzawa, Kazuyoshi Murata, Kouki Nishikawa, Kazutoshi Tani and Yoshinori Fujiyoshi,
Organizer
IGER International Symposium on Frontiers in Biological Research with Advanced Electron Microscope Technologies
Place of Presentation
Nagoya University
Year and Date
2015-01-15 – 2015-01-16
Related Report
Invited
-
-
-
-
