| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this research project, I have tried to reveal the entire extracellular region of sorLA (sorLAect: 2040 amino acids). SorLA is a single-pass transmembrane protein and is recognized as the potent risk factor of Alzheimer’s disease. At the beginning, it was technically impossible to obtain the recombinant sorLAect, and so I planed the structural study using the several divided portions of sorLAect. However, at the early stage of this project, I found the novel way to express the sorLAect by the co-expression method with a chaperon protein. Then I changed the plan for this project to establishing the expression and purification system of sorLAect for the single-molecule visualization using electron microscope. As a result, I successfully obtained the first negatively stained electron micrographs of sorLAect in the world, which showed that sorLAect takes an extended conformation without any sharp turns.
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