| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Outline of Final Research Achievements |
Recently, a novel transcription regulator CarH has been characterized as a cobalamin-dependent photosensor. Though it is reported that CarH regulates the biosynthesis of carotenoids in response to light, the detail molecular mechanisms of light sensing and functional regulation of CarH in response to light were unclear.
We determined the crystal structure of adenosylcobalamin-bound CarH from Thermus thermophilus.
CarH consists of the N-terminal DNA binding domain and the C-terminal sensor domain. The adenosyl group of adenosylcobalamin stabilizes the conformation of the sensor domain. We assumed that loss of adenosyl group upon photosensing results in change of the quaternary structure from tetramer to monomer.
|
