Direct observation of the ligand-dissociation dynamics of the hemoglobin

• Project/Area Number: 26840028
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Jichi Medical University (2015-2016); High Energy Accelerator Research Organization (2014)
• Principal Investigator: Sato Ayana 自治医科大学, 医学部, 助教 (50717709)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ヘモグロビン / 光解離 / 結晶構造解析 / 時間分解測定 / 配位子 / ラウエ回折

Outline of Final Research Achievements

To trace structural changes of carbonmonoxy hemoglobin associated with ligand photolysis, the X-ray crystallography under laser irradiation conditions were carried out. Using a set of coordinates of those intermediate states of hemoglobin, the quantitative conformation change in inner subunit and intra subunits of hemoglobin were appraised. The sequential structural change in each subunit propagated across the overall tetrameric molecule and produced the quaternary conformational motion.

Research Products

• [Journal Article] Structural Dynamics Measurements of the Biological Molecule Using Pump-Probe Technique (2014)
  • Author(s): A.Tomita, T.Sato, S.Nozawa, N.Shibayama and S.Adachi
  • Journal Title: Nihon Kessho Gakkaishi Volume: 56 Issue: 4 Pages: 253-258
  • DOI: 10.5940/jcrsj.56.253
  • NAID: 130004688358
  • ISSN: 0369-4585, 1884-5576
  • Peer Reviewed / Open Access
• [Presentation] X-RAY FLUORESCENCE HOLOGRAPHY FOR PROTEINS: APPLICATION TO HEMOGLOBIN AND MYOGLOBIN (2017)
  • Author(s): Ayana Sato-Tomita, Naohisa Happo, Sam-Yong Park, Koichi Hayashi, Yuji C. Sasaki, Naoya Shibayama
  • Organizer: Biophysical Society 61st Annual Meeting
  • Place of Presentation: New Orleans Ernest N. Morial Convention Center (United States of America)
  • Year and Date: 2017-02-11
  • Int'l Joint Research
• [Presentation] 生体分子活性サイトの原子分解能イメージング (2016)
  • Author(s): 佐藤文菜, 柴山修哉, 八方直久, 松下智裕, 朴三用, 林好一, 佐々木裕次
  • Organizer: 第10回 物性科学領域横断研究会
  • Place of Presentation: 神戸大学（兵庫県神戸市）
  • Year and Date: 2016-12-09
• [Presentation] Visualization by X-ray fluorescence holography of metal environments in hemoglobin (2016)
  • Author(s): Ayana Sato-Tomita, Naoya Shibayama, Naohisa Happo, Kouichi Hayashi, Yuji C. Sasaki
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2016-11-25
• [Presentation] New structural analysis methods for hemoglobin crystals. Time-resolved cryogenic X-ray crystallography with extended pulsed-laser pumping and 3D imaging by X-ray fluorescence holography (2016)
  • Author(s): Ayana Sato-Tomita, Shin-ichi Adachi, Sam-Yomg Park, Yuji C. Sasaki, Koichi Hayashi and Naoya Shibayama
  • Organizer: The Biophysical Society 60th Annual Meeting
  • Place of Presentation: Los Angeles
  • Year and Date: 2016-02-27
  • Int'l Joint Research
• [Presentation] レーザー光照射によるヘモグロビンの構造ダイナミクス研究 (2016)
  • Author(s): 佐藤文菜
  • Organizer: 第 29回日本放射光学会年・放射光科学合同シンポジウム
  • Place of Presentation: 柏の葉カンファレンスセンター
  • Year and Date: 2016-01-09
  • Invited
• [Presentation] Visualization of iron environments in hemoglobin using X-ray fluorescence holography (2016)
  • Author(s): Ayana Sato-Tomita, Naoya Shibayama, Naohisa Happo, Koji Kimura, Takahiro Okabe, Tomohiro Matsushita, Sam-Yong Park, Yuji C. Sasaki, Kouichi Hayashi
  • Organizer: Local 3D atomic and electronic structure imaging of functionally active sites
  • Place of Presentation: University of Zurich (Swiss Confederation)
  • Int'l Joint Research
• [Presentation] ヘモグロビンの配位子結合サイトから伝播する分子構造変形の直接観測 (2015)
  • Author(s): 佐藤文菜、柴山修哉、足立伸一、朴三用、林好一、佐々木裕次
  • Organizer: 第9回 物性科学領域横断研究会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-11-13
• [Presentation] Structural dynamics measurements of the intermediate states in the ligand-photolysis of hemoglobin (2015)
  • Author(s): Ayana Sato
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] ヘムタンパク質のリガンド解離過程における構造ダイナミクス実時間観測 (2015)
  • Author(s): 富田文菜、佐藤篤志、野澤俊介、柴山修哉、Kyung Hwan Kim、Hyotcherl Ihee、足立伸一
  • Organizer: 第3回物構研サイエンスフェスタ
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2015-03-17 – 2015-03-18
  • Invited
• [Presentation] 一酸化炭素型ヘモグロビンの光解離中間体の観測 (2014)
  • Author(s): 富田文菜，佐藤篤志，野口大貴，野澤俊介， 朴三用，柴山修哉，足立伸一
  • Organizer: 平成26年度日本結晶学会年会
  • Place of Presentation: 東京大学本郷キャンパス
  • Year and Date: 2014-11-01 – 2014-11-03
• [Presentation] X-ray crystal structures of carbonmonoxy hemoglobin photolysis intermediates (2014)
  • Author(s): 富田文菜、佐藤篤志、野口大貴、野澤俊介、腰原伸也、朴三用、柴山修哉、足立伸一
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27