| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
An intramembrane protease RseP is a key regulator in the σE-pathway extracytoplasmic stress response (ESR) which is critical for bacterial survival under stressed conditions. In this research project, I have aimed to understand a whole picture of the ESR system. Through a detailed functional analysis of the Escherichia coli RseP, I have revealed that two novel regions in RseP, the MRE β-loop and the C1N regions, play a crucial role for substrate recognition and cleavage mechanism. This finding gives us new insight into intramembrane cleaving proteases highly conserved from bacteria to human. We have identified some novel proteolytic substrates of RseP, which might taught us possible new roles of RseP. I have also established a live-cell imaging system for visualizing an RseP-dependent substrate cleavage event by fluorescent microscopy. This system must be useful for a kinetic analysis for the ESR system in the future.
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