Elucidation of pectin biosynthesis mechanism involving novel Golgi-localized polygalacturonase
Project/Area Number |
26840034
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
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Research Institution | Osaka University |
Principal Investigator |
OHASHI TAKAO 大阪大学, 生物工学国際交流センター, 助教 (10597876)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | ペクチン / 植物細胞壁 / ポリガラクツロナーゼ / 分裂酵母 / シロイヌナズナ / 細胞壁 / ゴルジ / GPIアンカー / 多糖分解酵素 / 糖質分解酵素 / タバコ培養細胞 |
Outline of Final Research Achievements |
Polygalacturonase is pectin-hydrolyzing enzyme. It was thought that the polygalacturonase was secreted and localized at cell wall, and degrade the assembled pectic polysaccharides. Especially the cell wall-type polygalacturonases involved in the fruit maturation, leave and fruit abscissions and pollen maturation were well studied. In this study, I found the novel Golgi-localized polygalacturonase and enzymatically characterized. In addition, I attempted to elucidate the physiological functions of the Golgi-localized polygalacturonase using Arabidopsis T-DNA mutants.
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Report
(4 results)
Research Products
(6 results)
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[Presentation] Novel membrane-bound type plant pectin-degrading enzyme2016
Author(s)
Takao Ohashi, Mami Suzuki, Nabilah Sari, Ryo Misaki, Kazuhito Fujiyama
Organizer
JSPS Japanese-German Graduate Externship International Symposium “Biological and Chemical Methods for Selective Catalysis”
Place of Presentation
RWTH Aachen University, Germany
Year and Date
2016-09-06
Related Report
Int'l Joint Research
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