Elucidation of pectin biosynthesis mechanism involving novel Golgi-localized polygalacturonase

• Project/Area Number: 26840034
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: OHASHI TAKAO 大阪大学, 生物工学国際交流センター, 助教 (10597876)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ペクチン / 植物細胞壁 / ポリガラクツロナーゼ / 分裂酵母 / シロイヌナズナ / 細胞壁 / ゴルジ / GPIアンカー / 多糖分解酵素 / 糖質分解酵素 / タバコ培養細胞

Outline of Final Research Achievements

Polygalacturonase is pectin-hydrolyzing enzyme. It was thought that the polygalacturonase was secreted and localized at cell wall, and degrade the assembled pectic polysaccharides. Especially the cell wall-type polygalacturonases involved in the fruit maturation, leave and fruit abscissions and pollen maturation were well studied. In this study, I found the novel Golgi-localized polygalacturonase and enzymatically characterized. In addition, I attempted to elucidate the physiological functions of the Golgi-localized polygalacturonase using Arabidopsis T-DNA mutants.

Research Products

• [Journal Article] A polygalacturonase localized in the Golgi apparatus in Pisum sativum (2017)
  • Author(s): Ohashi T, Jinno J, Inoue Y, Ito S, Fujiyama K, Ishimizu T
  • Journal Title: J Biochem Volume: 印刷中 Issue: 3 Pages: 193-201
  • DOI: 10.1093/jb/mvx014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 植物細胞壁研究のフロンティア (2015)
  • Author(s): 大橋貴生
  • Journal Title: 生物工学会誌 Volume: 93 Pages: 37-37
  • Peer Reviewed / Open Access
• [Presentation] Novel membrane-bound type plant pectin-degrading enzyme (2016)
  • Author(s): Takao Ohashi, Mami Suzuki, Nabilah Sari, Ryo Misaki, Kazuhito Fujiyama
  • Organizer: JSPS Japanese-German Graduate Externship International Symposium “Biological and Chemical Methods for Selective Catalysis”
  • Place of Presentation: RWTH Aachen University, Germany
  • Year and Date: 2016-09-06
  • Int'l Joint Research
• [Presentation] 高等植物由来推定GPIアンカー型ポリガラクツロナーゼの細胞内局在解析 (2016)
  • Author(s): 大橋貴生、鈴木真未、三﨑亮、藤山和仁
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌）
  • Year and Date: 2016-03-27
• [Presentation] Functional characterization of membrane-bound polygalacturonase from Arabidopsis thaliana (2016)
  • Author(s): １０９．Takao Ohashi, Mami Suzuki, Nabilah Sari, Muneyoshi Matsushita, Ryo Misaki, Kazuhito Fujiyama
  • Organizer: Biotechnology and Chemistry for Green Growth
  • Place of Presentation: Osaka University, Suita, Osaka, Japan
  • Year and Date: 2016-03-09
  • Int'l Joint Research / Invited
• [Presentation] 高等植物における膜結合型ポリガラクツロナーゼの酵素学的特性解析 (2015)
  • Author(s): 大橋貴生、ムスタファ ナビラー サリ、松下宗義、三﨑亮、藤山和仁
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学（岡山県岡山市）
  • Year and Date: 2015-03-26 – 2015-03-29