| Project/Area Number |
26840035
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
TERAHARA Naoya 大阪大学, 生命機能研究科, 招聘研究員 (40554738)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 生体分子モーター / 1分子計測 / べん毛モーター |
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| Outline of Final Research Achievements |
The rotational energy of flagellar motor is mediated by inward ion flux through the channel of the stator complexes. Bacillus subtilis has not only H+-channel MotAB but also Na+-channel MotPS as the stator. It is unknown that how the torque is generated by two distinct stators. Here, we measured the rotation speed of single motor by attaching the bead to flagellar filament under various conditions to see whether these two stators actually work together in a motor. In wild-type motors co-expressing MotAB and MotPS, the torque-speed curve was very similar to that of MotAB motors under Na+-free condition. However, the maximum speed was decreased with an increase in Na+ concentration, while the zero-speed torque was almost constant value. These observations indicate that functional incorporation of MotPS into the motor depend on the Na+ causes the speed reduction under low-load condition, but MotPS is capable of coordinately generating the torque with MotAB under high-load condition.
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