| Project/Area Number |
26840038
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | University of Miyazaki |
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Principal Investigator |
AKIEDA SAYAKA 宮崎大学, フロンティア科学実験総合センター, 助教 (20549076)
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| Co-Investigator(Renkei-kenkyūsha) |
DATE YUKARI 宮崎大学, 理事 (70381100)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | マクロファージ / 肥満 / 生理活性物質 / エネルギー代謝調節 / グアニリン / 脂肪細胞 / 生理活性ペプチド |
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| Outline of Final Research Achievements |
Recently, we showed that double-transgenic (dTg) rats overexpressing guanylin (Gn) and its receptor, GC-C, specifically in macrophages did not become obese even when fed a high-fat diet. In this study, to characterize macrophages expressing Gn and GC-C, we analyzed the expression of the M1 markers of macrophages isolated from dTg and wild type (WT) rats. The expression of proinflammatory cytokines and M1 macrophage markers were expressed at a significantly lower level in the macrophages of dTg rats than in those of WT rats. We also found that the chemotaxis of Gn/GC-C macrophages incubated with fatty acids significantly increases compared to the macrophages of WT rats. Our results suggest that the low levels of proinflammatory markers in Gn/GC-C macrophages at least in part contribute to the anti-obese phenotype of dTg rats. In addition, the accelerated chemotaxis of Gn/GC-C macrophages in response to fatty acids suggests that these macrophages can uniquely react to excess fatty acids.
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