Single-molecule characterization of target DNA search of tumor suppressor p53
| Project/Area Number |
26840045
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Tohoku University |
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Principal Investigator |
Kamagata Kiyoto 東北大学, 多元物質科学研究所, 助教 (90432492)
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| Research Collaborator |
SUGIYAMA HIROSHI 京都大学, 大学院理学研究科, 教授 (50183843)
ENDO MASAYUKI 京都大学, 物質-細胞統合システム拠点, 准教授 (70335389)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 蛋白質 / DNA / １分子計測 / １分子計測(SMD) / 遺伝子 / 単分子計測 / 天然変性蛋白質 / 探索 / 結合 |
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| Outline of Final Research Achievements |
p53 can preventDNA. the cancer of a living cell by binding to a part of DNA (target). In this study, we investigated the mechanism in which p53 searches and binds to the target and the role of disordered region of p53. We measured the movement of p53 along DNA. We found that p53 has a low target recognition probability（TRP） and regulates TRP by posttransmodifications of p53. Furhtermore, we found that elongation of the disordered region of p53 caused the loss of specific binding to DNA.
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] One-dimensional sliding of p53 along DNA is accelerated in the presence of Ca(2+) or Mg(2+) at millimolar concentrations2015
Author(s)
Murata A, Ito Y, Kashima R, Kanbayashi S, Nanatani K, Igarashi C, Okumura M, Inaba K, Tokino T, Takahashi S, Kamagata K
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Journal Title
JOURNAL OF MOLECULAR BIOLOGY
Volume: 427
Issue: 16
Pages: 2663-2678
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 一分子蛍光観測法によるがん抑制蛋白質p53のスライディング運動の解明2015
Author(s)
村田崇人, 伊藤優志, 五十嵐千裕, Dwiky Subekti Rendra Graha, 高橋聡, 鎌形清人
Organizer
The 53rd Annual Meeting of the Biophysical Society of Japan
Place of Presentation
金沢
Year and Date
2015-09-13
Related Report
Int'l Joint Research
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[Presentation] Target Search Process of A Tumor Suppressor p53 Revealed by Single-Molecule Fluorescence Microscopy2014
Author(s)
Murata A., Itoh, Y., Kashima, R., Tokino, T., Takahashi, S., Kamagata, K.
Organizer
The 16th international p53 workshop
Place of Presentation
Stockholm, Sweden
Year and Date
2014-06-15 – 2014-06-19
Related Report
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