| Project/Area Number |
26840054
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Japan Atomic Energy Agency |
|---|
Principal Investigator |
Sunami Tomoko 国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究副主幹 (50554648)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Keywords | 転写因子 / タンパク質・DNA相互作用 / Bacterial One-Hybrid法 / B1Hアッセイ / DNA / ホメオドメイン |
|---|
| Outline of Final Research Achievements |
Our purpose is to develop proteins with properties different from widely used genome-editing proteins such as TALEN and CRISPR. In this study, we rationally designed novel proteins based on engrailed homeodomain and confirmed the binding specificity using bacterial one-hybrid assay. We successfully designed molecules with a strong affinity and high specificity for the target sequence which has two engrailed homeodomain binding sites. Also, we developed a method to evaluate molecular flexibility of DNA using electron density map of X-ray crystal structures.
|
