Uncovering operating principle of bio-molecular system by simultaneous observation of dynamics of multiple individual molecules
| Project/Area Number |
26840057
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Ichimura Taro 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (50600748)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 単分子イメージング / 生物物理 / 一分子計測 |
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| Outline of Final Research Achievements |
Simultaneous nanometric tracking of multiple motor proteins was achieved by combining multicolor fluorescent labeling of target proteins and imaging spectroscopy, revealing dynamic behaviors of multiple motor proteins at the sub-diffraction-limit scale. Using quantum dot probes of distinct colors, we experimentally verified the localization precision to be a few nanometers at temporal resolution of 30 ms or faster. One-dimensional processive movement of two heads of a single myosin molecule and multiple myosin molecules was successfully traced. Furthermore, the system was modified for two-dimensional measurement and applied to tracking of multiple myosin molecules. Our approach is useful for investigating cooperative movement of proteins in supramolecular nanomachinery.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it.2016
Author(s)
Takamitsu J. Morikawa, Hideaki Fujita, Akira Kitamura, Takashi Horio, Johtaro Yamamoto, Masataka Kinjo, Akira Sasaki, Hiroaki Machiyama, Keiko Yoshizawa, Taro Ichimura, Katsumi Imada, Takeharu Nagai & Tomonobu M. Watanabe
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 22342-22342
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 波長分離による複数分子ナノ追跡2015
Author(s)
市村垂生, 垣塚太志, 池崎圭吾, 金城純一, 藤田英明, 渡邉朋信
Organizer
第56回応用物理学会年会
Place of Presentation
名古屋国際会議場（愛知県・名古屋市）
Year and Date
2015-09-13
Related Report
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