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Analysis of ER-associated degradation components by multiple knockout cell lines using TALEN

Research Project

Project/Area Number 26840065
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionKyoto University (2015)
Okazaki Research Facilities, National Institutes of Natural Sciences (2014)

Principal Investigator

Ninagawa Satoshi  京都大学, 理学(系)研究科(研究院), 研究員 (80647991)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsEDEM / mannose / N-glycan / 小胞体関連分解 / mannose trimming / 糖鎖 / ERAD / 小胞体 / マンノーストリミング
Outline of Final Research Achievements

Glycoproteins misfolded in the endoplasmic reticulum (ER) are subjected to ER-associated glycoprotein degradation. In this process, N-glycan mannose trimming is required. We clearly showed that this process is initiated by EDEM2 and completed by EDEM3/EDEM1.
We also demonstrated that higher eukaryotes are able to extract severely misfolded glycoproteins from glycoprotein ERAD and target them to the non-glycoprotein ERAD pathway to maintain the homeostasis of the ER.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (2 results)

  • [Journal Article] Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway.2015

    • Author(s)
      Ninagawa S, Okada T, Sumitomo Y, Horimoto S, Sugimoto T, Ishikawa T, Takeda S, Yamamoto T, Suzuki T, Kamiya Y, Kato K, Mori K.
    • Journal Title

      The Journal of Cellular Biology

      Volume: 211 Issue: 4 Pages: 775-784

    • DOI

      10.1083/jcb.201504109

    • NAID

      120005672576

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 糖鎖依存的構造異常タンパク質分解に必須な糖鎖刈り込み機構を解明 革新的ゲノム編集技術によって従来のモデルを一新2015

    • Author(s)
      蜷川 暁、加藤 晃一、森 和俊
    • Journal Title

      化学と生物

      Volume: Vol.53, No.9 Pages: 571-573

    • NAID

      130005405061

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step.2014

    • Author(s)
      Ninagawa S, Okada T, Sumitomo Y, Kamiya Y, Kato K, Horimoto S, Ishikawa T, Takeda S, Sakuma T, Yamamoto T, Mori K
    • Journal Title

      J Cell Biol.

      Volume: 206 Issue: 3 Pages: 347-356

    • DOI

      10.1083/jcb.201404075

    • NAID

      120005468381

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] EDEM1/2/3 are α1,2-mannosidases essential for endoplasmic reticulum-associated degradation of glycoproteins2014

    • Author(s)
      Satoshi Ninagawa, Tetsuya Okada, Yoshiki Sumitomo, Yukiko Kamiya, Satoshi Horimoto, Tokiro Ishikawa, Shunichi Takeda, Tetsushi Sakuma, Takashi Yamamoto, Koichi Kato, Kazutoshi Mori
    • Organizer
      SFG JSCR Joint Meeting
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2014-11-16 – 2014-11-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 小胞体で新生タンパク質に付加されたN型糖鎖からのマンノーストリミングはEDEM2によって開始される2014

    • Author(s)
      蜷川暁、岡田徹也、住友嘉樹、神谷由紀子、堀本賢、石川時郎、武田俊一、佐久間 哲史、山本卓、加藤晃一、森和俊
    • Organizer
      第33回日本糖質学会
    • Place of Presentation
      愛知県名古屋市 名古屋大学
    • Year and Date
      2014-08-10 – 2014-08-12
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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