AMP-activated protein kinase regulates the function of tight junction.

Research Project

Project/Area Number	26840068
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Cell biology
Research Institution	Osaka University
Principal Investigator	Tomoki Yano 大阪大学, 医学(系)研究科(研究院), 助教 (20546121)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	細胞間接着 / タイトジャンクション / 細胞骨格 / AMP活性化プロテインキナーゼ / アドヘレンスジャンクション / AMP活性プロテインキナーゼ
Outline of Final Research Achievements	I found that the AMP-activated protein kinase (AMPK) phosphorylates several proteins of tight junction (TJ). I showed that when cingulin, it is tight junctional protein, was phosphorylated by AMPK, the molecular configurational change occurred. By exploiting this conformational alteration regulated by AMPK, I try to design a module that undergoes efficient fluorescence resonance energy transfer (FRET). FRET probe is possible to be a powerful tool to visualize the intracellular nutritional condition by observing the activity of AMPK.